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Oxford Handbook of RNA Viruses: A Practical Approach
Oxford University Press, June 2000, Pages: 288
- Provides an overview of fundamentals in RNA virus research.
- Covers many specialized techniques for studying viruses.
- Over 120 protocols and many figures.
- Essential volume for all laboratories involved in RNA virus research.
RNA Viruses: A Practical Approach is wide ranging in scope, from emerging technology such as reverse genetics and retrovirus vectors, to money saving tips - how to make your own silica particles for high efficiency RNA extraction and liposomes for cell transfection! Chapter one covers the fundamentals of investigating RNA virus genome structure at a molecular level. Chapters two and three describe techniques for mutagenesis of RNA genomes and analysis of transcription. Chapter four deals with RNA virus-encoded proteinases, an important aspect of the control of RNA virus gene expression. Chapter five considers retrovirus oncogenesis and chapter six analysis of RNA virus quasispecies. Chapter seven describes systems for investigation of in vitro replication of positive-stranded viruses and chapter eight the packaging of RNA virus genomes. In addition to the technical aspects of reverse genetics and retrovirus vectors, both of the final two chapters also consider ethical aspects of these new technologies.
Readership: Industrial virus research laboratories, public health laboratories and academia. Postgraduates reading biology and medicine.
About the Author
Edited by Alan Cann, Department of Microbiology and Immunology, University of Leicester
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