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Current Protocols Essential Laboratory Techniques
John Wiley and Sons Ltd, June 2008, Pages: 806
This report provides the reader with both the understanding and the skills necessary to become a productive member of the modern life science laboratory. The latest title from the acclaimed series of Current Protocols laboratory manuals, it is both a teaching tool meant to serve as the primary text in an advanced undergraduate laboratory class or biological technician training program, and a practical reference manual for the life science researcher.
The book begins by covering basic skills such as measurement (e.g., mass, volume, pH, radioactivity), preparation of reagents, and use of basic instrumentation, then seamlessly guides the reader into more advanced techniques such as blotting, electrophoresis, and real-time PCR. The book also includes appendices detailing the proper keeping of a laboratory notebook (including information regarding intellectual property issues), instructions for creating posters from programs such as PowerPoint, and detailed explanations of common figure file formats and when to use them. In order to allow the reader to understand the basis for the method and what steps are required before the experiment is performed, every chapter describing a technique includes Overview and Principles and Strategic Planning sections in conjunction with clear step-by-step protocols. Chapters also include sections such as Understanding Results and Troubleshooting to assist the reader in data interpretation and accounting for unexpected results. References to more advanced uses of the technique are given in the Variations section of each chapter.
Written by a combination of distinguished researchers and outstanding teaching faculty, this report is the cornerstone on which the beginning researcher can develop the skills of a successful research career.
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