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Practical Cell Analysis
John Wiley and Sons Ltd, Feb 2010, Pages: 320
As analytical chemistry and biology move closer together, biologists are performing increasingly sophisticated analytical techniques on cells. Chemists are also turning to cells as a relevant and important sample to study newly developed methods. Practical Cell Analysis provides techniques, hints, and time-saving tips explaining what may be “common knowledge” to one field but are often hidden or unknown to another.
Within this practical guide:
- The procedures and protocols for cell separation, handling cells on a microscope and for using cells in microfluidic devices are presented.
- Elements of cell culture are taken and combined with the practical advice necessary to maintain a cell lab and to handle cells properly during an analysis
- The main chapters deal with the fundamentals and applied aspects of each technique, with one complete chapter focusing on statistical considerations of analyzing cells
- Many diagram-based protocols for some of the more common cell processes are included
Chapter summaries and extensive tables are included so that key information can be looked up easily in the lab setting
Much like a good manual or cookbook this book is a useful, practical guide and a handy reference for all students, researchers and practitioners involved in cellular analysis.
This book serves as a guide for researchers interested in adding cell analyses to their repertoire. This book could also be kept in labs of groups currently performing cell analyses as a handy reference. It will cover the basics of setting up a cell culture-capable laboratory, as well as sources and types of cells. Rather than focus solely on cell culture techniques, it will also discuss the procedures/protocols for cell separation, handling cells on a microscope (for maximum viability, etc), and for using cells in microfluidic devices. Cell viability, probe toxicity, apoptosis, cell storage, and other practical stumbling blocks will be discussed. Each chapter will have useful tables such as common cell fluorescence probes, separation methods/protocols, etc. The book will also discuss in detail the statistical considerations of analyzing cells (which in turn dictate how some experiments are performed). Above all, this book will be written with a practical emphasis at all times, so that it will have a widespread audience and will remain close at hand in the laboratory
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