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Molecular Biological and Immunological Techniques and Applications for Food Chemists
John Wiley and Sons Ltd, Jan 2010, Pages: 492
A guide to using molecular biology and immunological methods for the analysis of food
This book is a good resource and guide for chemists and food chemists in particular, with interest in the analysis of regulated complex analytes such as food allergens and bioengineered foods from food samples by using both or either molecular biology and/or immunoassays. Many of the analytical problems that food chemists face in the lab cannot be solved by chemistry alone, and so chemists increasingly turn to the techniques used for molecular biology and immunology--techniques in which they have not necessarily received training. This book presents up-to-date international regulatory position of several organic food contaminants and tracking systems to identify and prevent adulteration practices. Coverage includes state-of-the-art molecular biology techniques and immunoassays that support regulatory agencies in enforcing regulations and the industry to comply. The in-depth description of both types of assays covers not only practical elements from the laboratory point of view, but also pitfalls usually overseen and potential solutions. Detailed applications of these techniques include the detection of food allergens, veterinary residues, bioengineered crops, and targets used in speciation and tracking of fraudulent practices.
Many of the analytical problems that food chemists face in the lab cannot be solved by chemistry alone, and so analytical chemists are turning to molecular biology and immunology for alternative approaches. Molecular Biological and Immunological Techniques and Applications for Food Chemists comprehensively explains the most important molecular biology and immunology methods, and illustrates their application in food analysis.
Written by a distinguished group of experts, the coverage includes:
- Molecular Biological Methods—techniques explained, laboratory layout, PCR, real-time PCR, RFLP, SSCP, and sequencing
- Molecular Biology Applications—meat, genetically modified organisms (GMOs), food allergens, offal, and fish
- Immunological Methods—techniques explained and antibody-based detection methods
- Immunology Applications—animal speciation, international food allergen regulations (except Japanese), Japanese regulations and buckwheat allergen detection, egg allergen detection, soy allergen detection, milk allergen detection, gluten allergen detection, nut allergen detection, fish allergen detection, lupin allergen detection, mustard allergen detection, and celery allergen detection
Clearly written and consistently edited to provide information to a wide range of readers, Molecular Biological and Immunological Techniques and Applications for Food Chemists offers an up-to-date reference for food scientists in government and industry, policymakers, and graduate-level students of food science, technology, and engineering.
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