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Assay Development: Fundamentals and Practices
John Wiley and Sons Ltd, May 2010, Pages: 429
Essential principles and practice of assay development
The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting.
Rather than attempting comprehensive coverage of all assay development technologies, the book introduces the most widely used assay development technologies and illustrates the art of assay development through a few commonly encountered biological targets in assay development (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). Just enough biological background for these biological targets is provided so that the reader can follow the logics of assay development.
Chapters discuss:
- The basics of assay development, including foundational concepts and applications
- Commonly used instrumental methods for both biochemical assays and cell-based assays
- Assay strategies for protein binding and enzymatic activity
- Cell-based assays
- High-throughput screening
An in-depth study of the now popular Caliper's off-chip kinase assay provides an instructive, real-world example of the assay development process.
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