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Practical High-Performance Liquid Chromatography, 5th Edition
John Wiley and Sons Ltd, April 2010, Pages: 426
This textbook aims to present the knowledge which is necessary for everyday HPLC measurements to be made, in a detailed and easy-to-follow manner. It contains a wealth of practical material and covers a wide range of subjects. Topics covered include principles, instrumentation, reverse-phase, ion-exchange, ion-pair, ion, size-exclusion and affinity chromatography, as well as analytical methods, preparative HPLC and enantiomeric separations. Appendices provide information on instrument testing, troubleshooting, column-packing.
Jump into the HPLC adventure!
New sections on the following topics have been included in this fifth edition:
- Comparison of HPLC with capillary electrophoresis
- How to obtain peak capacity
- van Deemter curves and other coherences
- Hydrophilic interaction chromatography
- Method transfer
- Comprehensive two-dimensional HPLC
- Fast separations at 1000 bar
- HPLC with superheated water
In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added.
A completely new chapter is presented on quality assurance covering:
- Is it worth the effort?
- Verification with a second method
- Method validation
- Standard operating procedures
- Measurement uncertainty
- Qualifications, instrument test, and system suitability test
- The quest for quality
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