CELL BIOLOGY ASSAYS

Elsevier Science and Technology, November 2009, Pages: 394

Cell Biology Assays: Protein presents a comprehensive overview of protocols essential for research in proteins.  Chapters provide clear explanations, step-by-step instruction, tips and potential pitfalls.  The important steps and results are illustrated for further ease of use. This text enables researchers at various career levels a plethera of insight into technologies and model systems in biological problem solving.

- Focus on protein identifcation using mass spectrometry
- Step-by-step procedures detailing materials, procedures, comment and pitfalls
- Information on the plethora of technologies needed to tackle complex problems

Protein Determination
Phosphopeptide Mapping: A Basic Protocol
Coupling of Fluorescent Tags to Proteins
Radioiodination of Proteins and Peptides
Free-Flow Electrophoresis
Gel-Based Proteomics: High-Resolution Two-Dimensional Gel Electrophoresis of Proteins. Isoelectric Focusing and Nonequalibrium pH Gradient Electrophoresis.
High-Resolution Two-Dimensional Electrophoresis with Immobilized pH Gradients for Proteome Analysis
Two-Dimensional Difference Gel Electrophoresis: Application for the Analysis of Differential Protein Expression in Multiple Biological Samples
Affinity Electrophoresis for Studies of Biospecific Interations: High-Resolution Two-Dimensional Affinity Electrophoresis for Separation of Hapten-Specific Polyclonal Antibodies into Monoclonal Antibodies in Murine Blood Plasma
Image Analysis and Quantitation
Fluorescence Detection of Proteins in Gels Using SYPRO Dyes
Autoradiography and Fluorography: Film-Based Techniques for Imaging Radioactivity in Flat Samples
Two-Dimensional Gel Profiling of Posttanslationally Modified Proteins by in vivo Isotope Labeling
Immunoprecipitation of Proteins under Nondenaturing Conditions
Nondenaturing Polyacrylamide Gel Electrophoresis as a Method for Studying Protein Interations: Applications in the Analysis of Mitochrondrial Oxidative Phosphorylation Complexes
Affinity purification with Natural Immobilized Ligands
Analysis of Protein-Protein Interactions by Chemical Cross-Linking
Peroxisomal Targeting as a Tool to Assess Protein-Protein Interactions
Biomolecular Interaction Analysis Mass Spectrometry
Blot Overlays with P-Labedled GST-Ras Fusion Proteins: Application to Mapping Protein-Protein Interaction Sites
Ligand Blot Overlay Assay: Detetion of Ca+2- and Small GTP-Binding Proteins
Modular Scale Yeast Two-Hybrid Screening
Chromophore-Assisted Laser Inactivation of Proteins by Antibodies Labeled with Malachite Green
Chromatin Immunoprecipitation (ChIP)
Gel Mobility Shift Assay
DNA Affinity Chromatography of Transcription Factors: The Oligaonucleotide Trapping Approach
Protein Degradation Methods: Chaperone-Mediated Autophagy
Methods in Protein Ubiquitination
Protein Identification and Sequencing by Mass Spectrometry
Proteome Specific Sample Preparation Methods for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
In-Gel Digestion of Protein Spots for Mass Spectrometry
Peptide Sequencing by Tandem Mass Spectrometry
Direct Database Searing Using Tandem Mass Spectra of Peptides
Identification of Proteins from Organisms with Unsequenced Genomes by Tandem Mass Spectrometry and Sequence-Similarity Database Searching Tools
Identification of Protein Phosphorylation Sites by Mass Spectrometry
Analysis of Carbohydrates/Glycoproteins by Mass Spectrometry
Stable Isotope Labeling by Amindo Acids in Cell Culture for Quantitative Proteomics
Site-Specific, Stable Isotopic Labeling of Cysteinyl Pepties in Complex Peptide Mixtures
Protein Hydrogen Exchange measured by Electrospry Ionization Mass Spectrometry
Nongel Based Proteomics: Selective Reversed-Phase Chromatographic Isolation of Methionine-Containing Peptides from Complex Peptide Mixtures
Mass Spectrometry in Noncovalent Protein Interactions and Protein Assemblies

Geri, Kreitzer

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