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Analytical Determination of Beta-lactam Antibiotics. Edition No. 1

Description:

This study was focused to develop an analytical method to analyze beta-lactam antibiotics in aqueous solutions. The method is based on the chemical reaction between beta-lactam antibiotics with phosphomolybdic acid, a heteropolyacid able to be reduced by the thiols obtained after the acid hydrolysis of the beta-lactam antibiotics. The blue product obtained, which is proportional to the antibiotic amount, is adsorbed on polyurethane foam, and the intensity of color is directly evaluated on the solid material by means of diffuse reflectance spectroscopy.

For the evaluated beta-lactam antibiotics, the analytical parameters achieved were: LOD = 0.03 µg/ml, LOQ = 0.1 µg/ml, RSD = 4.0 %. The study of possible interferences showed that using solid phase extraction is possible to eliminate even strong interferences as ascorbic acid, cysteine and reducing salts like stannous chloride.

The method has avoided, as many as possible, the use of expensive and contaminating solvents and chemical reagents. The method presents the possibility to be applied using only a desktop scanner and a personal computer for measuring the intensity of color of the adsorbed material on the polyurethane foam.

Author

Roman Rodriguez Maecker.
BSc in Chemistry at Escuela Superior Politecnica de Chimborazo (ESPOCH), Ecuador (1999). Doctor rer. nat.: Environmental Analytical Chemistry at University Duisburg - Essen, Germany (2006). Docent and researcher at Universidad Tecnica de Ambato (UTA), Ecuador. His main field of interest is the protection of the water resources in Ecuadorian Andes.

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Analytical Determination of Beta-lactam Antibiotics. Edition No. 1

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Analytical Determination of Beta-lactam Antibiotics. Edition No. 1

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Order information

Product name

Web Address

Office Code

Report Formats

Contact information

Please fax this form to: (646) 607-1907 or (646) 964-6609 (from USA) +353-1-481-1716 or +353-1-653-1571 (from Rest of World)

Payment information

Please fax this form to: (646) 607-1907 or (646) 964-6609 (from USA) +353-1-481-1716 or +353-1-653-1571 (from Rest of World)

Please fax this form to:
(646) 607-1907 or (646) 964-6609 (from USA)
+353-1-481-1716 or +353-1-653-1571 (from Rest of World)

Please fax this form to:
(646) 607-1907 or (646) 964-6609 (from USA)
+353-1-481-1716 or +353-1-653-1571 (from Rest of World)