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Measurement of Free Radicals and their Effects on Human Spermatozoa. Edition No. 1

Description:

We presented data on the role of free radicals in
human spermatozoa, particularly in the context of
centrifugation.Methods were developed to directly
measure intracellular free radicals in human sperm
and the effects of exogenously applied free radicals
on sperm function were established. The role of
brief and prolonged centrifugation and the
associated generation of free radicals was also
investigated.In the first part of the study, we
established flow cytometry as a reliable tool for
directly measuring intracellular free radicals in
human spermatozoa. In the second part of the study
the effects of centrifugation on free radical
generation in sperm was investigated. The effects of
exogenously administered free radicals on sperm
function were investigated in the third part of the
study.In this study, novel techniques have been
developed to successfully measure free radicals in
human spermatozoa.We recommend that cognisance
should be taken of the potentially adverse effects
of both centrifugation and free radicals on sperm
function with the ultimate goal of improving the
outcome of assisted reproductive technologies.

Author

Fanuel Lampiao.
Mr. Fanuel Lampiao is a PhD student since 2006 in reproductive
biology at the Division of Medical Physiology of Stellenbosch
University under the supervision of Dr Stefan du Plessis. His
research interests are on the effects of reactive oxygen species
as well as insulin and leptin on male fertility.

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Measurement of Free Radicals and their Effects on Human Spermatozoa. Edition No. 1

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Report Formats

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Please fax this form to: (646) 607-1907 or (646) 964-6609 (from USA) +353-1-481-1716 or +353-1-653-1571 (from Rest of World)

Payment information

Please fax this form to: (646) 607-1907 or (646) 964-6609 (from USA) +353-1-481-1716 or +353-1-653-1571 (from Rest of World)

Please fax this form to:
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+353-1-481-1716 or +353-1-653-1571 (from Rest of World)

Please fax this form to:
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+353-1-481-1716 or +353-1-653-1571 (from Rest of World)