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Viewing report
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Biotechnology in Agriculture. Edition No. 1
VDM Publishing House, Oct 2008, Pages: 104
Twelve mango cultivars grown in the Egypt were
discriminated by their seed SDS-protein and PCR-
based molecular markers. For PCR analysis three
different
techniquess were used including novel technique.
Comparitive analysis were conductied beween the two
old technique, ISSR and RAPD, and the novel
tecnique, three primers-based RAPD. The cultivar-
specific markers represented 24.46%of the total
markers (regardless of type of analysis), 88.89% of
them were RAPD markers. Most of these markers were
scored for the presence of unique bands. Cultivar-
specific markers were shown to be useful in
constructing linkage map that involve any
polymorphic gene(s). Dendrogram tree generated
across SDS-protein, RAPD, ISSR and three promers-
based analysis. Constructed dendrograms for results
,individually or collectively, revealed that
similarity and clustering is dependant on the
marker system used.
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