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Viewing report
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Coiled Coil Interfaces for Nanoscale Amperometric
Biosensors. Edition No. 1
VDM Publishing House, Oct 2008, Pages: 260
Both gold nanostructures and carbon nanotubes are
rapidly emerging platforms for amperometric biosensor
detection. Homogeneous display of biomolecular
ligands is a key process in forming nanoscale array
biosensors, improving the chances for sufficient
signal strength and reproducibility at the limits of
sensor geometries. The use of self-assembling
interfaces on gold electrodes and CNT arrays enables
homogeneous display, solvent access, target capture,
sensor surface regeneration and potential for
molecular wiring. Our approach uses an anchor
peptide, covalently linked to the sensor surface,
that is able to non-covalently capture probe labeled
targets in solution with a high affinity and minimal
surface leaching. The use of non-disrupting,
metal-chelating scaffolds within the coiled coil
sequence was tested for molecular wiring capabilities
using model redox targets. Recombinant cassette
production of probe sequences was investigated as a
means to enable control over spatial and
stoichiometric placement of redox active mediators
and/or biomolecules. This thesis defines potential
challenges as well as opportunities for future
developments.
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