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Viewing report
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Molecular Characterisation of the Ribgrass Mosaic
Virus - Impatiens. Edition No. 1
VDM Publishing House, Oct 2008, Pages: 112
Regarding a strategy of expressing foreign genes in
plants, two diverged RMV-based expression vectors
containing a green fluorescent protein were cloned
into the binary vector pPZP200 for subsequent
agroinfiltration work. An infectious full-length
clone of RMV and three RMV-based mutants already
existed. They vary depending on which of the two
putative ORFs is inactivated. An additional mutation
site of one of these mutation constructs was
discovered and reverse mutated. After the inoculation
of the constructs on N. benthamiana and N. tabacum,
the analysis revealed distinct effects in virus
accumulation and symptom characteristics of the three
mutation constructs. These results differ from the
ones of the wild type clone. Accordingly, all three
mutants show both lower virus accumulation levels and
lesser symptom characteristics. Further, the results
from N. benthamiana indicate an ability to reverse
mutate the -4K virus. In addition to the description
and the analysis of these results, the potential role
of the two putative ORFs will be discussed and an
outlook on further investigations will be given.
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