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Activation tagging in transgenic poplar. Edition No. 1

VDM Publishing House, August 2010, Pages: 116

We describe the establishment of an activation tagging system in poplar based upon the maize Ac/Ds transposable element system. Transgenic poplar plants were obtained carrying either one or two of the above mentioned constructs - Following induction of the GIP promoter only a weak transposase signal could be detected in RT- PCR experiments. Mobility of the ATDs activation construct could not be confirmed in the GIP system. - An Activation Tagging experiment was initiated using eight different double transgenic lines. A few putative variants could be obtained revealing deficiency in chlorophyll content. - Single transgenic aspen lines carrying HSP- transposase have been produced. Following transformation with HSP-transposase a chimerical transgenic line was obtained revealing white and green plants. Following activation tagging experiment a HSP-transposase/ATDs-rolC double transgenic line, one somaclonal variation line was obtained revealing white plants.

Fadia, El Sherif.
Fadia El Sherif, ph-D, Lecturer and Researcher at Suez Canal University, Ismailia, Egypt. Hamdy Kamal Atta-Alla, ph-D, Professor of Horticulture at Suez Canal University, Ismailia, Egypt. Matthias Fladung, ph-D, PD, Group leader and vice-head of the Johann Heinrich von Thuenen Institute, Institute for Forest Genetics, Germany.
Hamdy, Kamal.
Fadia El Sherif, ph-D, Lecturer and Researcher at Suez Canal University, Ismailia, Egypt. Hamdy Kamal Atta-Alla, ph-D, Professor of Horticulture at Suez Canal University, Ismailia, Egypt. Matthias Fladung, ph-D, PD, Group leader and vice-head of the Johann Heinrich von Thuenen Institute, Institute for Forest Genetics, Germany.
Matthias, Fladung.
Fadia El Sherif, ph-D, Lecturer and Researcher at Suez Canal University, Ismailia, Egypt. Hamdy Kamal Atta-Alla, ph-D, Professor of Horticulture at Suez Canal University, Ismailia, Egypt. Matthias Fladung, ph-D, PD, Group leader and vice-head of the Johann Heinrich von Thuenen Institute, Institute for Forest Genetics, Germany.