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Metabolic regulation analysis based on gene and protein expressions. Edition No. 1

Description:
The biopolymer poly-3-hydroxybutyrate (PHB) is a polyester produced by certain bacteria utilizing glucose, corn starch or wastewater nutrients. The characteristics of PHB are similar to those of petroplastic polypropylene. PHB produces transparent film at a melting point higher than 130 degree Celsius that is non toxic and biodegradable without residues, which reduce greenhouse gas emissions considerably. Currently, application of PHB is limited to medical science because of its higher production cost. Microbial biosynthesis of PHB starts with the condensation of two acetyl-CoA molecules into acetoacetyl-CoA, which is subsequently reduced to hydroxybutyryl-CoA. This latter compound is then used as a monomer to polymerize PHB. To explore the possibilities for maximum PHB production, this book has investigated E. coli metabolic regulations by inserting PHB synthetic genes and/or deleting glycolytic pathway genes together with changing their culture condition. Study results from proteomics, transcriptomics, measurement of key enzyme activities and metabolic flux analysis have been interpreted for better understanding of their complex genotype-phenotype relationship.
 
Author
MUKHTAR, AHMED.
I have conducted studies on fatty acid composition in maize under drought conditions up to graduation level. Specialized in resource conservation technologies to mitigate effects of the water crisis/drought causing economic loss to the agriculture and now working on climate change and simulation modeling.
., Fayyaz-ul-Hassan.
I have conducted studies on fatty acid composition in maize under drought conditions up to graduation level. Specialized in resource conservation technologies to mitigate effects of the water crisis/drought causing economic loss to the agriculture and now working on climate change and simulation modeling.
Mustazhar-Naseeb, Akram.
I have conducted studies on fatty acid composition in maize under drought conditions up to graduation level. Specialized in resource conservation technologies to mitigate effects of the water crisis/drought causing economic loss to the agriculture and now working on climate change and simulation modeling.
 
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Metabolic regulation analysis based on gene and protein expressions. Edition No. 1

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