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A Guide to Green-Fluorescent Protein
Drug and Market Development Publishing, March 2003, Pages: 200
This resource-oriented practical Guide provides an all encompassing overview of the most important features and technological applications of Green-Fluorescent Protein and its variant forms. It seeks to describe Green-Fluorescent Protein's usefulness in biomedical programs and applications.
Proteomics and genomics have provided a rich supply of targets in drug discovery programs. However, selecting and understanding both drugs and targets in the context of the living cell are critical to early evaluation of drug action and efficacy. This challenge has given rise to the development of cellular and molecular probes of drug action and a new emphasis on 'cellomics.'
One probe gaining in popularity is the Green Fluorescent Protein (GFP). As a fluorescent probe, its rare genetic encoding enables both real-time and non-invasive reporting in living cells. This ability provides a basis for cell-based monitoring of GFP-linked targets upon administration of external drugs. The impact of GFP has been revolutionary. GFP has not just facilitated visualization of intricate cellular architecture but it has also acted as a marker of protein dynamics and behavior in cell biology. These applications have been translated to drug discovery where, GFP, and its color variants have been utilized in fluorescence and confocal imaging, HTS/HCS screening assays and in in vivo diagnostics. GFP can not only be used in early stage target characterization but also in retrieving non-invasive 'whole organism' data and in evaluating lead compound toxicology.
This Guide will:
-Demonstrate the value of GFP applications in cell biology-which have accelerated GFP implementation into drug discovery programs that relate to living cells.
Illustrate how GFP is currently being used in the biopharmaceutical industry.
-Evaluate many of the available GFP and 'GFP-like' variants and present their strengths and weaknesses.
Review the instruments suitable for GFP detection.
This Guide will Prove Beneficial to:
-Directors of Research & Development and Business Development at Pharmaceutical, Biotechnology, CRO's, and Diagnostic companies.
-In addition, this Guide will prove invaluable to Group Leaders, Senior Scientists, Post-Doctoral and Technical Researchers within Research Institutions, Government Research Institutes (biology), and Universities.
-Also expected to benefit are Directors of Research & Development and Business Development within companies that provide molecular and drug discovery tools and fluorescent detection instruments.
-This Guide could also be useful for Educators, Instructors, and Lecturers who provide GFP/biotechnology workshops and continuing education courses.
This Guide presents an overview of the most important features of Green-Fluorescent Protein (GFP) and is an invaluable, practical resource for:
-Those not currently using GFP it will serve as a valuable introduction to the potential uses of Green-Fluorescent Protein.
-Those using GFP it explains how to maximize data acquisition and data interpretation.
-Those interested in cloning, expressing and purifying GFP it provides some useful considerations and pointers.
This Guide will answer the following questions:
-What questions need to be asked when evaluating a new GFP variant?
-What measures can be taken in creating a successful GFP fusion?
-What are the common problems encountered in monitoring GFP fluorescence?
-Where does GFP fit in to drug discovery?
-How can I use GFP in multicolor labeling experiments?
-How can I avoid false positives or false negatives in GFP-based assays?
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