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Correlative Light and Electron Microscopy IV. Methods in Cell Biology Volume 162

  • Book

  • March 2021
  • Elsevier Science and Technology
  • ID: 5146456

Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more.

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Table of Contents

1. Workflow (dis)-advantages / spiderwebOri Avinoam2. Serial section LM + EMErin M. Tranfield3. Platinum clusters as CLEM probesPaul Verkade4. Correlative Light Electron Microscopy with a transition metal complex as a single probePaul Verkade5. Detection EDX + EELSBen N. G. Giepmans6. SEM-TEM-SIMSLouise Jensen7. HPF-CLEMXavier Heiligenstein8. A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyesThomas M?ller-Reichert9. Three-dimensional on-section correlative light and electron microscopy of large cellular volumes using STEM tomographyKorbinian Buerger10. Accelerated procedure for approaching and imaging of optically branded ROI in tissueNatalia V. Gounko11. Volume-CLEMKedar Narayan12. Cryo SOFI CLEMRainer Kaufman13. Cryo fluo after Cryo lamellaPetr Chlanda14. Super resolution CLEMS?lvia Pujals15. FIB-SEM + softwareAllon Weiner16. Correlia, CLEM softwareMatthias Schmidt17. Correlated Multimodality Imaging beyond CLEM: From in-vivo Preclinical Imaging to ex-vivo MicroscopyAndreas Walter18. Community effortsPaul Verkade