Enzymes in RNA Science and Biotechnology, Volume 691 in the Methods in Enzymology series, highlights new advances in the field, including chapters on Reverse transcriptase Part I (discovery, preparation, general utilization, MarathonRT for routine RT-PCR and for cDNA synthesis on challenging RNA templates Structured RNAs, repeat RNAs and more, Engineering TNA Polymerases Through Iterative Cycles of Directed Evolution, Reverse transcriptase Part II (RNA structure mapping and determination), RNA G-quadruplex (rG4) structure detection using RTS and SHALiPE assays, tRNA Structure-seq in vivo and in droplets, Capture the in vivo intact RNA structurome by CAP-STRUCTURE-seq, and more.
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Table of Contents
Reverse transcriptase Part I (discovery, preparation, general utilization)1. MarathonRT for routine RT-PCR and for cDNA synthesis on challenging RNA templates Structured RNAs, repeat RNAs and more2. Engineering TNA Polymerases Through Iterative Cycles of Directed EvolutionReverse transcriptase Part II (RNA structure mapping and determination)3. RNA G-quadruplex (rG4) structure detection using RTS and SHALiPE assays4. tRNA Structure-seq in vivo and in droplets5. Capture the in vivo intact RNA structurome by CAP-STRUCTURE-seq6. Sequencing-based analysis of RNA structures in living cells with SHAPE-MaPRNA polymerase (discovery, preparation, development/engineering and application)7. Making RNA: Using T7RNA polymerase to produce high yields of RNA from DNA templates8. A Simple Approach to Improving RNA Synthesis: Salt Inhibition of RNA Rebinding Coupled with Strengthening Promoter Binding by a Targeted Gap in the DNA9. Isolation of E. coli RNA polymerase transcription elongation complexes by selective photoreversible solid-phase immobilizationRNA ligase10. RNA ligation using vaccinia RNA ligase enzyme: high yield RNA coupling for RNA circularization, adapter ligation and other applications11. Chemical crosslinking and ligation methods for in vivo analysis of RNA structures and interactionsPost-transcriptional modification enzymes 12. Genome-wide detection of dihydrouridine modifications in RNA13. BID-seq: The quantitative base-resolution method for pseudouridines in mammalian mRNA.14. Quantitative DAMM-seq maps multiple RNA methylations in tRNA and mitochondrial dsRNA15. Discovering RNA modification enzymes by a comparative genomics approach16. Functional analysis of tRNA modification enzymes using Mutational profiling17. Fluorescent labelling of tRNA for interaction studies with RNA modification enzymes18. Synthesis of long RNA with a site-specific modification by enzymatic splint ligationEndo/Exo nucleases 19. Characterizing RNase L-mediated mRNA decay in single cells20. Characterization of RNase J21. DICER cleavage assays22. 23. DROSHA cleavage assays
