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Single Cell Analysis: Technologies and Applications


Description: This first modern book on the topic gives a broad overview on current technology and application areas for single cell approaches in life sciences.

Identification and imaging of single cells which is the most widely applied single cell technology, is described in the opening section, including fluorescence, electron tomography and atomic force techniques. The core section of the book covers a wide range of technologies for the handling, manipulation and constituent analysis of individual cells. The final section is dedicated to case studies and specific applications, with examples ranging from cell biology and genetics to molecular medicine.

With its wide range to technologies and applications, this book is the perfect companion for all scientists entering this growing field.


Contents: SINGLE CELL ANALYSIS: IMAGING
Single Molecule Fluorescence Monitoring in Eukaryotic Cells: Intranuclear Dynamics of Splicing Factors
Gene Classification and Quantitative Analysis of Gene Regulation in Bacteria using Single Cell Atomic Force Microscopy and Single Molecule Force
Cellular Cryo-Electron Tomography (CET): Towards a Voyage to the Inner Space of Cells

SINGLE CELL ANALYSIS: TECHNOLOGY
Single Cell Proteomics
Protein Analysis of Single Cells in Microfluidic Format
Single Cell Mass Spectrometry
Single-Cell Analysis for Quantitative Systems Biology
Optical Stretcher for Single Cells

SINGLE CELL ANALYSIS: APPLICATIONS
Single Cell Immunology
Molecular Characterization of Rare Single Tumor Cells
Single Cell Heterogeneity
Genome and Transcriptome Analysis of Single Tumour Cells


Author Dario Anselmetti holds the chair in Experimental Biophysics and Applied Nanosciences at Bielefeld University (Germany). He obtained his academic degrees in Physics, Mathematics and Astronomy from Basel University (Switzerland), before becoming a post-doc at the IBM Research Laboratories. Later on, he worked for six years in the research department of Ciba-Geigy (now Novartis) as a project leader in nanobiophysics. After co-managing the Solvias spin-off company in Basel, he returned to academia, taking up his current position in 2000. Dr. Anselmetti has authored over 100 scientific publications and is a member of the Academy of Sciences of the state of Nordrhein-Westfalen.


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