• 1-800-526-8630U.S. (TOLL FREE)
  • 1-917-300-0470EAST COAST U.S.
  • +353-1-416-8900REST OF WORLD
E-Learning Course - Microbiology Laboratory Practices (Certified) Product Image

E-Learning Course - Microbiology Laboratory Practices (Certified)

  • ID: 310171
  • March 2014
  • Skillpad

This course covers the key critical techniques and practices that are required knowledge for all competent microbiologists.

It covers basic microbiological techniques such as media preparation, pure culture techniques and the pour plate technique. The application of good aseptic technique to microbiological testing is described in detail.

A key tool in microbiology – the microscope – is comprehensively covered by explaining its main components and key microscopic techniques.

The main categories of staining – simple, differential, and structural – are described along with the different staining techniques commonly used in a microbiology laboratory. Finally, the concept of unknown bacterial identification is explained and the morphological and cultural tests carried out to identify unknowns.

This course consists of the following modules:

- Principles of Good Aseptic Technique
- Basic Microbiological Techniques
- Introduction to Microscopy
- Introduction to Staining
- Staining Techniques
- Unknown Bacterial Identification

Certificate

There is a 16 question assessment associated with each lesson within the Course and we offer a Certificate Service in which Users who achieve 100% in the assessment associated with each Lesson of a Course are deemed to have passed the Course, and are posted a Certificate.

Note: Product cover images may vary from those shown

(1) Principles of Good Aseptic Technique

This Skillpad Micro laboratory Module is ideal for both new and existing Laboratory employees in the Pharmaceutical, Biotech, and Contract sectors. The Module explains the importance of good aseptic technique and the major steps involved in applying it to microbiological testing.

Objectives:

- Describe how to flame an inoculating loop
- Describe the correct aseptic technique for handling agar plates
- Describe the correct aseptic technique for handling liquid broths
- Describe the general steps for reducing the risk of contamination in microbiological testing
- Explain the terms "false positive" and "positive control"
- Explain why good aseptic technique is necessary
- List the three common steps that apply to all microbiological handling techniques

(2) Basic Microbiological Techniques

This Skillpad Micro laboratory Module is ideal for both new and existing Laboratory employees in the Pharmaceutical, Biotech, and Contract sectors. The Module explains the techniques used frequently by microbiologists including media preparation, pure culture techniques and the pour plate technique.

Objectives:

- Describe common streak plate method mistakes
- Describe how reserve and working stock cultures are used and stored
- Describe the pour plate method
- Describe the quadrant and continuous streak plate methods
- Explain the difference between agar plates, agar slants and agar deeps
- Explain the difference between reserve and working stock cultures
- Explain the term "microbiological media" and name the three different media forms

(3) Introduction to Microscopy

This Skillpad Micro laboratory Module is ideal for both new and existing Laboratory employees in the Pharmaceutical, Biotech, and Contract sectors. The Module explains the importance of microscopy in microbiology. The main components of a microscope and microscopic techniques.

Objectives:

- Describe the correct handling procedure of microscopes
- Describe the role of microscopy in microbiology
- Discuss the different microscopy techniques available to the microbiologist, including bright-field, phase contrast and dark-field illumination
- Explain how the lens system functions
- List the different lenses in the lens system
- Name and explain the different components of a microscope

(4) Introduction to Staining

This Skillpad Micro laboratory Module is ideal for both new and existing Laboratory employees in the Pharmaceutical, Biotech, and Contract sectors. The Module explains how to prepare and heat fix a smear correctly. Introduction to three main categories of staining i.e. simple, differential and structural.

Objectives:

- Describe the different bacterial stains – Simple, Differential and Structural
- Explain the procedure for heat fixing bacterial smears
- Explain the procedure for preparing smears from liquid and solid cultures
- List and describe examples of smearing errors
- List the different categories of bacterial stains

(5) Staining Techniques

This Skillpad Micro laboratory Module is ideal for both new and existing Laboratory employees in the Pharmaceutical, Biotech, and Contract sectors. The Module explains the different staining techniques commonly used in a microbiology laboratory.

Objectives:

- Describe the purpose and procedure of acid-fast and spore staining and list examples of the dyes used
- Describe the purpose and procedure of gram staining and list examples of the dyes used
- Describe the purpose and procedure of simple and negative staining and list examples of the dyes used
- Explain the terms "blotting", "cover", "decolorize" and "stain"
- List common gram staining problems

(6) Unknown Bacterial Identification

This Skillpad Micro laboratory Module is ideal for both new and existing Laboratory employees in the Pharmaceutical, Biotech, and Contract sectors. The Module introduces the concept of unknown bacterial contamination and the morphological and cultural tests carried out to identify these unknowns.

Objectives:

- Describe how bacteria are named
- Describe the purpose and procedure of simple and negative staining and list examples of the dyes used
- Describe what is involved in morphological & cultural testing & what information is obtained
- Explain the term "unknown bacteria"
- List the categories of microbiological identification tests

Note: Product cover images may vary from those shown
Note: Product cover images may vary from those shown

ALSO AVAILABLE

RELATED PRODUCTS

Our Clients

Our clients' logos