- Presents detailed protocols
- Includes troubleshooting tips
- Provides coverage on structural biology, computational methods, and biology
Chapter 1: Purification of Polyglutamine Proteins
Chapter 2: Preparation of amyloid beta-protein for structural and functional studies.
Chapter 3: Kinetics and thermodynamics of amyloid assembly using an HPLC-based sedimentation assay. Chapter 4: Protein aggregation starting from the native, globular stete.
Chapter 5: Direct observation of amyloid growth monitored by total internal reflection fluorescence microscopy.
Chapter 6: Characterization of Amyloid Structures at the Molecular Level by Solid State Nuclear Magnetic Resonance Spectroscopy.
Chapter 7: Spin Labeling Analysis of Amyloids and other Protein Aggregates.
Chapter 8: Hydrogen-Deuterium Exchange Mass Spectrometry of Protein Aggregates.
Chapter 9: Hydrogen-Deuterium Exchange analyzed by Matrix Assisted Laser Desorption-Ionisation Mass
Spectrometry and the HET-s prion model.
Chapter 10: Analysis of amyloid fibril structure by scanning cysteine mutagenesis.
Chapter 11: Sedimentation velocity analysis of amyloid oligomers and fibrils.
Chapter 12: Structural study of metastable amyloidogenic protein oligomers by Photo-Induced Cross-linking of Unmodified Proteins (PICUP).
Chapter 13: High Pressure Studies on Protein Aggregates and Amyloid Fibrils.
Chapter 14: Phage Display Screening for Peptides that Inhibit Polyglutamine Aggegation.
Chapter 15: Peptide Based Inhibitors of Amyloid Assembly.
Chapter 16: Screening for Modulators of Aggregation with a Microplate Elongation Assay.
Chapter 17: Conformation-dependent anti-amyloid oligomer antibodies.