This third volume in the series covers such topics as anaesthetics, cannulation and injection techniques, and surgery. The book will be invaluable to fisheries scientists, aquaculturists, and animal biochemists, physiologists and endocrinologists; it will provide researchers and students with a pertinent information source from theoretical and experimental angles.
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catecholamines (S.D. Reid, S.F. Perry). Membrane preparation and receptor assays
adenosine (J.F. Siebenaller, T.F. Murray). Fish mitochondria (J.S. Ballantyne). Peroxisomes and peroxisomal &Bgr;-oxidation (C.D. Moyes, E.L. Crockett). Use of membrane vesicle techniques to characterize nutrient transport processes of the teleost gastrointestinal tract (G.A. Ahearn, C. Storelli). Incorporation of 35S-sulphate into branchial cartilages: a biological model to study hormonal regulation of skeletal growth in fish (C. Duan). Development of radioimmunoassay for a model peptide hormone: insulin (E.M. Plisetskaya). Radioimmunoassay for growth hormones, prolactins and somatolactins (P. Swanson). Hormone measurement by ELISA: application to fish gonadotrophin (O. Kah). Developing an ELISA for a model protein
vitellogenin (J.L. Specker, T.R. Anderson). Bolus-injection of radiolabels in the study of steady state glucose metabolism (T.G. West). In vivo nuclear magnetic resonance spectroscopy of aquatic animals (A. van Waarde, G. van den Thillart). Analysis of enzyme regulation via reversible phosphorylation and enzyme binding interactions with macromolecules (K.B. Storey). Metabolite transport in fish red blood cells and hepatocytes (T.W. Moon, P.J. Walsh). Ca2+ transport across plasma membranes (G. Flik, P.M. Verbost). Analyzing phosphatidylinositol cycling and protein kinase C involvement (F.W. Goetz). Appendix: Buffered salt solutions, culture media and tissue homogenization buffers (T.P. Mommsen, P.W. Hochachka). Species Index. Subject Index.