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Caenorhabditis Elegans, Vol 106. Edition No. 2. Methods in Cell Biology

  • ID: 1757869
  • Book
  • December 2011
  • 504 Pages
  • Elsevier Science and Technology
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An updated edition of the classic Methods in Cell Biology volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.

  • Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to come
  • Chapters written by leaders in the field
  • Tried and true methods deliver busy researchers a one-stop compendium of essential protocols

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Culturing methods and genetic procedures General methods of culture: Jim Lewis Mutagenesis: Phil Anderson Genetic screening methods: James Thomas, Eric Jorgensen, Eric Lambie Genetic mapping methods: Ben Williams, Stephen Wicks (Boston College) Genetic mosaic analysis: Robert Herman Use of specialized chromosomes and rearrangements: Ann Rose, David Baillie Gene knockouts: Shohei Mitani, Robert Barstead RNAi and RNAi screening methods: Julie Ahringer, Andrew Dillin

Genomics and Bioinformatics Transcriptional profiling: Stuart Kim, Don Moerman, Valerie Reinke MicroRNA methods: Victor Ambros, Frank Slack, Amy Pasquinelli Analysis of RNA function: Marv Wickens, Judith Kimble Analysis of the interactome: Marc Vidal WormBase: Eric Schwartz, Paul Sternberg, Lincoln Stein Structural genomics: Ming Luo, Larry DeLucas, Songlin Li, Mike Carson (U. Alabama) Proteomics: Ahna Skop, Moerman D., Isobe T, Krijgsveld, J Transcriptional regulatory networks: Marion Walhout

Markers for gene expression and protein localization Immunofluorescence and antibodies: David Miller Imaging: John White, William Mohler Electron Microscopy: David Hall Proteins and protein assemblies: Henry Epstein Analysis of morphogenesis: Jeff Hardin, Andrew Chisholm Cell death: Monica Driscoll, Ding Xue

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Rothman, Joel.

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