An updated edition of the classic Methods in Cell Biology volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.
- Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to come
- Chapters written by leaders in the field
- Tried and true methods deliver busy researchers a one-stop compendium of essential protocols
Culturing methods and genetic procedures General methods of culture: Jim Lewis Mutagenesis: Phil Anderson Genetic screening methods: James Thomas, Eric Jorgensen, Eric Lambie Genetic mapping methods: Ben Williams, Stephen Wicks (Boston College) Genetic mosaic analysis: Robert Herman Use of specialized chromosomes and rearrangements: Ann Rose, David Baillie Gene knockouts: Shohei Mitani, Robert Barstead RNAi and RNAi screening methods: Julie Ahringer, Andrew Dillin
Genomics and Bioinformatics Transcriptional profiling: Stuart Kim, Don Moerman, Valerie Reinke MicroRNA methods: Victor Ambros, Frank Slack, Amy Pasquinelli Analysis of RNA function: Marv Wickens, Judith Kimble Analysis of the interactome: Marc Vidal WormBase: Eric Schwartz, Paul Sternberg, Lincoln Stein Structural genomics: Ming Luo, Larry DeLucas, Songlin Li, Mike Carson (U. Alabama) Proteomics: Ahna Skop, Moerman D., Isobe T, Krijgsveld, J Transcriptional regulatory networks: Marion Walhout
Markers for gene expression and protein localization Immunofluorescence and antibodies: David Miller Imaging: John White, William Mohler Electron Microscopy: David Hall Proteins and protein assemblies: Henry Epstein Analysis of morphogenesis: Jeff Hardin, Andrew Chisholm Cell death: Monica Driscoll, Ding Xue