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Cryo-EM, Part C, Vol 483. Methods in Enzymology

  • ID: 1759205
  • Book
  • 380 Pages
  • Elsevier Science and Technology
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This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

* Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide

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  1. Analyses of Subnanometer Resolution Cryo-EM Density Maps

    Matthew L. Baker, Mariah R. Baker Corey F. Hryc Frank DiMaio

  2. Methods for Segmentation and interpretation of cryo-EM data

    Niels Volkmann

  3. Integration of Cryo-EM with atomic and protein: protein interaction data

    Friedrich Forster

  4. Unified Data Resource for CryoEM

    Catherine L. Lawson

  5. Electron crystallography and aquaporins

    Thomas Walz, Andreas D. Schenk, Richard K. Hite, Andreas Engel, Yoshinori Fujiyoshi

  6. Cryo-Electron Microscopy Applications in the Study of Tubulin Structure, Microtubule Architecture, Dynamics and Assemblies, and Interaction of Microtubules with Motors

    Kenneth H. Downin and Eva Nogales

  7. Helical crystallization of two example membrane proteins: MsbA and the Ca2+-ATPase

    Howard S. Young, John Paul Glaves, Lauren Fisher, Andrew Ward

  8. Multi-particle cryo-EM of ribosomes

    Christian M.T. Spahn, Justus Loerke, Jan Giesebrecht

  9. Single-particle electron microscopy of animal fatty acid synthase: Describing macromolecular rearrangements that enable catalysis

    Francisco Asturias, Edward J. Brignole

  10. Tomography of actin cytoskeletal networks

    Dorit Hanein

  11. Visual Proteomics

    Friedrich Förster, Bong-Gyoon Han, and Martin Beck

  12. Cryo-Electron Tomography of Eukaryotic Cells

    Ohad Medalia, Asaf Mader , Nadav Elad

  13. 3d Visualization Of Hiv Virions By Cryo Electron Tomography

    Jun Liu, Elizabeth R. Wright, Hanspeter Winkler

  14. Automation in Single-Particle Electron Microscopy: Connecting the Pieces

Bridget Carragher, Dmitry Lyumkis, Arne Moeller, Anchi Cheng, Amber Herold, Eric Hou, Christopher Irving, Erica L. Jacovetty, Pick-Wei Lau, Anke Mulder, James Pulokas, Joel D. Quispe, Neil R. Voss, Clinton S. Potter

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Jensen, Grant.

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