- Provides detailed information on expression systems- Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs- Includes timelines and examples- Compares pros and cons of each method
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Introduction: The Art of Expression.
Purpose of This Book.
Selecting a Suitable Expression System:
Genomics and the Future of Protein Expression Systems, J.M. Fernandez and J.P. Hoeffler.
Prokaryotic Expression Systems:
Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse, R. Durbin.
Expression Vectors Employing the trc Promoter, J. Brosius.
Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller.
araB Expression System in Escherichia coli, M. Better.
Eukaryotic Expression Systems:
Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow.
Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg.
Recombinant Protein Expression in Pichia methanolica, C.K. Raymond.
Cytomegalovirus Promoter for Expression in Mammalian Cells, M.F. Stinski.
Inducible Mammalian Expression Systems, M.Russell.
Protein Expression in Mammalian Cells Using Sindbis Virus, R.P. Bennett.
Expression in Insect Systems:
Drosophila S2 System for Heterologous Gene Expression, R.B. Kirkpatrick and A. Shatzman.
Baculovirus Expression Vector System, M. Galleno and A.J. Sick.
Recombinant Protein Expression in Transgenic Mice, R. Abbud and J.H. Nilson.
Expression of Recombinant Proteins in the Milk of Transgenic Animals, H.M. Meade, Y. Echelard, C.A. Ziomek, M.W. Young, M. Harvey, E.S. Cole, S. Groet, T.E. Smith, and J.M. Curling.
Recombinant Protein Expression in Plants, A.E. Voloudakis, Y. Yin, and R.N. Beachy.
Joseph Fernandez is the Vice President of Business Development at Invitrogen Corporation in Carlsbad, California, where he identifies and licenses new technologies including gene expression systems.
Hoeffler, James P.
Jim Hoeffler is the Director of Research and Development at Invitrogen Corporation, concentrating on the development of new products, with a focus on gene expression systems.