Sphingolipid Metabolism and Cell Signaling, Part B, Vol 312. Methods in Enzymology

  • ID: 1763596
  • Book
  • 688 Pages
  • Elsevier Science and Technology
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This volume contains information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. Its companion Volume 311 presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sphingolipids and analogs.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

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Section I: Methods for Analyzing Sphingolipids

[1]: Analysis of Sphingoid Bases and Sphingoid Base 1-Phosphates by High-Performance Liquid Chromatography

[2]: Enzymatic Method for Measurement of Sphingosine 1-Phosphate

[3]: Ceramide Mass Analysis by Normal-Phase High-Performance Liquid Chromatography

[4]: Quantitative Determination of Ceramide Using Diglyceride Kinase

[5]: Analysis of Sphingomyelin, Glucosylceramide, Ceramide, Sphingosine, and Sphingosine 1-Phosphate by Tandem Mass Spectrometry

[6]: Analyses of Glycosphingollpids by High-Performance Liquid Chromatography

[7]: Sphingolipid Extraction and Analysis by Thin-Layer Chromatography

[8]: Extraction and Analysis of Multiple Sphingolipids from a Single Sample

[9]: Purification of Sphingolipid Classes by Solid-Phase Extraction with Aminopropyl and Weak Cation Exchanger Cartridges

[10]: Ganglioside Analysis by High-Performance Thin-Layer Chromatography

[11]: Purification and Analysis of Gangliosides

[12]: Thin-Layer Chromatography Blotting Using Polyvinylidene Difluoride Membrane (Far-Eastern Blotting) and Its Applications

[13]: Thin-Layer Chromatography Immunostaining

[14]: Monoclonal Anti-Glycosphingolipid Antibodies

[15]: Immunolocalization of Gangliosides by Light Microscopy Using Anti-Ganglioside Antibodies

[16]: Cloud-Point Extraction of Gangliosides using Nonionic Detergent C14EO6

[17]: Analyses of Glycosphingolipids Using Clam, Mercenaria mercenaria, Ceramide Glycanase

[18]: Quantitative Analyses of Binding Affinity and Specificity for Glycolipid Receptors by Surface Plasmon Resonance

[19]: Use of Circular Dichroism for Assigning Stereochemistry of Sphingosine and Other Long-Chain Bases

[20]: Infrared Determination of Conformational Order and Phase Behavior in Ceramides and Stratum Corneum Models

[21]: Use of Nuclear Magnetic Resonance Spectroscopy in Evaluation of Ganglioside Structure, Conformation, and Dynamics

[22]: Fluorescence Quenching Assay of Sphingolipid/Phospholipid Phase Separation in Model Membranes

Section II: Methods for Analyzing Aspects of Sphingolipid Metabolism in Intact Cells

[23]: Synthesis of Fluorescent Substrates and Their Application to Study of Sphingolipid Metabolism in Vitro and in Intact Cells

[24]: Selection of Mammalian Cell Mutants in Sphingolipid Biosynthesis

[25]: Selection of Yeast Mutants in Sphingolipid Metabolism

[26]: Fluorescence-Based Selection of Gene-Corrected Hematopoietic Stem and Progenitor Cells Based on Acid Sphingomyelinase Expression

[27]: Mammalian Ganglioside Sialidases: Preparation and Activity Assays

Section III: Sphingolipid-Protein Interactions and Cellular Targets

[28]: Effects of Sphingosine and Other Sphingolipids on Protein Kinase C

[29]: Kinetic Analysis of Sphingoid Base Inhibition of Yeast Phosphatidate Phosphatase

[30]: Assays of Sphingosine-Dependent Kinase for 14-3-3 Protein

[31]: Synthesis and Use of Caged Sphingolipids

[32]: Binding of Sphingosine 1-Phosphate to Cell Surface Receptors

[33]: Use of Short-Chain Ceramides

[34]: Analysis of Ceramide-Activated Protein Phosphatases

[35]: Use of Affinity Chromatography and TID-Ceramide Photoaffinity Labeling for Detection of Ceramide-Binding Proteins

[36]: Lectin-Mediated Cell Adhesion to Immobilized Glycosphingolipids

[37]: Analysis of Glycolipid-Dependent Cell Adhesion Based on Carbohydrate-Carbohydrate Interaction

[38]: Analysis of Interactions between Glycosphingolipids and Microbial Toxins

[39]: Oxidation of Aglycone of Glycosphingolipids: Serine and Ceramide Acid Precursors for Soluble Glycoconjugates

[40]: Separation of Glycosphingolipid-Enriched Microdomains from Caveolar Membrane Characterized by Presence of Caveolin

[41]: Reconstitution of Sphingolipid-Cholesterol Plasma Membrane Mlcrodomalns for Studies of Virus-Glycolipid Interactions

[42]: Analysis of Ceramides Present in Glycosylphosphatidylinositol Anchored Proteins of Saccharomyces cerevisiae

[43]: Preparation of Functionalized Lipid Tubules for Electron Crystallography of Macromolecules

Section IV: Sphingolipid Transport and Trafficking

[44]: Applications of BODIPY-Sphingolipid Analogs to Study Lipid Traffic and Metabolism in Cells

[45]: Using Biotinylated Gangliosides to Study Their Distribution and Traffic in Cells by Immunoelectron Microscopy

[46]: Assays for Transmembrane Movement of Sphingolipids

Section V: Other Methods

[47]: Compilation of Methods Published in Previous Volumes of Methods in Enzymology

Author Index

Subject Index

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Abelson, John N.
Simon, Melvin I.
Merrill, Jr., Alfred H.
Hannun, Yusuf A.

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