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Heterotrimeric G Proteins, Vol 237. Methods in Enzymology

  • ID: 1765950
  • Book
  • 561 Pages
  • Elsevier Science and Technology
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This volume of Methods in Enzymology and its companion Volume 238 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins (Volume 237) and their effectors (Volume 238). Methods unique to signal transducing G proteins and general techniques applied to the study of G protein systems are covered.

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Ga Subunits:T. Wieland and K.H. Jakobs, Measurement of Receptor-Stimulated Guanosine 5'-O-((-Thio)triphosphate Binding by G Proteins.P. Gierschik, T. Bouillon, and K.H. Jakobs, Receptor-Stimulated Hydrolysis of Guanosine 5'-Triphosphate in Membrane Preparations.E.M. Ross and T. Higashijima, Regulation of G-Protein Activation by Mastoparans and Other Cationic Peptides.D.J. Carty and R. Iyengar, Guanosine 5'-O-((-Thio)triphosphate Binding Assay for Solubilized G Proteins.J. Moss, R.S. Haun, S.-C. Tsai, C.F. Welsh, F.-J.S. Lee, S.R. Price, and M. Vaughan, Activation of Cholera Toxin by ADP-Ribosylation Factors: 20-kDa Guanine Nucleotide-Binding Proteins.D.J. Carty, Pertussis Toxin-Catalyzed ADP-Ribosylation of G Proteins.R.R. Vaillancourt, N. Dhanasekaran, and A.E. Ruoho, Synthesis and Use of Radioactive Photoactivatable NAD+ Derivatives as Probes for G-Protein Structure.M.M. Rasenick, M. Talluri, and W.J. Dunn, III, Photoaffinity Guanosine 5'-Triphosphate Analogs as a Tool for the Study of GTP-Binding Proteins.L. Birnbaumer, D. Grenet, F. Ribeiro-Neto, and J. Codina, Preparation of Activated ( Subunits of Gs and Gis: From Erythrocyte to Activated Subunit.T. Katada, K. Kontani, A. Inanobe, I. Kobayashi, Y. Ohoka, H. Nishina, and K. Takahashi, Purification and Separation of Closely Related Members of Pertussis Toxin-Substrate G Proteins.J. Bigay and M. Chabre, Purification of Transducin.E. Lee, M.E. Linder, and A.G. Gilman, Expression of G-Protein a Subunits in Escherichia coli.I.-H. Pang, A.V. Smrcka, and P.C. Sternweis, Synthesis and Applications of Affinity Matrix Containing Immobilized (( Subunits of G Proteins.J.L. Blank and J.H. Exton, Purification of Activated and Heterotrimeric Forms of Gq Proteins.G.L. Waldo, J.L. Boyer, and T.K. Harden, Purification of Phospholipase C-Activating G Protein, G11, from Turkey Erythrocytes.J.R. Hepler, T. Kozasa, and A.G. Gilman, Purification of Recombinant Gqa, G11a, and G16a from Sf9 Cells.S.G. Graber, R.A. Figler, and J.C. Garrison, Expression and Purification of G-Protein a Subunits Using Baculovirus Expression System.E.J. Neer, B.M. Denker, T.C. Thomas, and C.J. Schmidt, Analysis of G-Protein a and (Subunits by in Vitro Translation.Y. Audigier, Assays for Studying Functional Properties of in Vitro Translated Gsa Subunit.S.M. Mumby and M.E. Linder, Myristoylation of G- Protein a Subunits.G. Milligan, Specificity and Functional Applications of Antipeptide Antisera Which Identify G-Protein a Subunits.K.-L. Laugwitz, K. Spicher, G. Schultz, and S. Offermanns, Identification of Receptor-Activated G Proteins: Selective Immunoprecipitation of Photolabeled G-Protein a Subunits.J. Lyons, Identification of Mutant Forms of G-Protein a Subunits in Human Neoplasia by Polymerase Chain Reaction-Based Techniques.P.V. Gejman and L.S. Weinstein, Detection of Mutations and Polymorphisms of the Gsa Subunit Gene by Denaturing Gradient Gel Electrophoresis.S. Winitz, M. Russell, and G.L. Johnson, Construction of Mutant and Chimeric G-Protein a Subunits.T.M. Wilkie, A.M. Aragay, A.J. Watson, and M.I. Simon, Design of Degenerate Oligonucleotide Primers for Cloning of G-Protein a Subunits.C. Kleuss, G. Schultz, and B. Wittig, Microinjection of Antisense Oligonucleotides to Assess G-Protein Subunit Function.R.M. Mortensen and J.G. Seidman, Inactivation of G-protein Genes: Double Knockout in Cell Lines.U. Rudolph, A. Bradley, and L. Birnbaumer, Targeted Inactivation of the Gi2( Gene with Replacement and Insertion Vectors: Analysis in a 96-Well Plate Format.B.E. Snaar-Jagalska and P.J.M. Van Haastert, G-Protein Assays in Dictyostelium.R.A. Cerione, Fluorescence Assays for G-Protein Interactions.H.E. Hamm and H.M. Rarick, Specific Peptide Probes for G-Protein Interactions with Receptors.F. Quan and M. Forte, Vaccinia Virus Systems for Expression of G( Genes in S49 Cells.
Gbg Subunits:J. Bigay and M. Chabre, Purification of Tbg Subunit of Transducin.J. Chen, D.J. Carty, and R. Iyengar, Adenylyl Cyclase Assay for bg Subunits of G Proteins.J. Dingus, M.D. Wilcox, R. Kohnken, and J.D. Hildebrandt, Synthesis and Use of Biotinylated bg Complexes Prepared from Bovine Brain G Proteins.C. Gallagher and N. Gautam, Design of Oligonucleotide Probes for Molecular Cloning of b and g Subunits.A.N. Pronin and N. Gautam, Characterization of Antibodies for Various G-Protein b and g Subunits.J.D. Robishaw and E.A. Balcueva, Preparation, Characterization, and Use of Antibodies with Specificity for G-Protein g Subunits.B.K.-K. Fung, J.S. Anant, W.-C. Lin, O.C. Ong, and H.K. Yamane, Isoprenylation of g Subunits and G-Protein Effectors.
Author Index.
Subject Index.
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Abelson, John N.
Simon, Melvin I.
Iyengar, Ravi.

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