This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process - or stops it - is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; MicroRNA expression, maturation and functional analysis; and MicroRNAs and disease.
Chapter 1: Identification of viral miRNAs
Chapter 2: Robust machine learning algorithms predict microRNA genes and targets
Chapter 3: Idenfication of virally encoded microRNAs
Chapter 4: Computational Methods for microRNA Target Prediction
SECTION II. Micro RNA expression, maturation and functional analysis
Chapter 5: In vitro and in vivo assays for the activity of the Drosha-DGCR8 complex
Chapter 6: Microarray analysis of microRNA gene expression.
Chapter 7: Cloning and detecting signature microRNAs from mammalian cells
Chapter 8: Approaches for studying microRNA and small interfering RNA methylation in vitro and in vivo
Chapter 9: Analysis of small RNA profiles during development.
Chapter 10: Dissecting MicroRNA-mediated Gene Regulation and Function in T Cell Development
SECTION III. MiRNAs and Disease
Chapter 11: Investigation of microRNAs alterations in leukemias and lymphomas
Chapter 12: Discovery of pathogen-regulated small RNAs in plants
Chapter 13: Protocols for expression and functional analysis of viral microRNAs