Microtubules: in vivo, Vol 97. Methods in Cell Biology

  • ID: 1768193
  • Book
  • 552 Pages
  • Elsevier Science and Technology
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Microtubules: in vivo includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation.

  • Includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton
  • The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation
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  1. Microtubule dynamics at the cell cortex probed by TIRF microscopy

    Ilya Grigoriev and Anna Akhmanova

  2. Microscopy methods for the study of centriole biogenesis and function in Drosophila

    Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica Bettencourt-Dias

  3. Quality control in single molecule studies of kinesins and microtubule-associated proteins

    Gary J. Brouhard

  4. Imaging cilia in zebrafish

    Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and Rebecca D. Burdine

  5. Automatic Identification of Microtubules in Cellular Electron Tomography

    Daniyar Nurgaliev, Timur Gatanov and Daniel J. Needleman

  6. Imaging and analysis of the microtubule cytoskeleton in Giardia

    Scott C. Dawson and Susan A. House

  7. Kinetochore-microtubule dynamics and attachment stability

    Jennifer G. DeLuca

  8. Assessment of mitotic spindle phenotypes in Drosophila S2 cells

    Gohta Goshima

  9. Live cell imaging techniques for analyses of microtubules in Dictyostelium

    Matthias Samereier, Irene Meyer, Michael P. Koonce and Ralph Gräf

  10. Microtubule dynamics in dendritic spines

    Lukas C. Kapitein , Kah Wai Yau and Casper C. Hoogenraad

  11. Analysis of Microtubules in Budding Yeast

    Alexander Rauch, Elena Nazarova and Jackie Vogel

  12. Determination of Microtubule Dynamic Instability in Living Cells

    Kathy Kamath, Emin Oroudjev, and Mary Ann Jordan

  13. Modeling microtubule-mediated forces and centrosome positioning in Caenorhabditis elegans embryos

    Akatsuki Kimura1 and Shuichi Onami

  14. Cryo electron tomography of cellular microtubules

    Roman I. Koning

  15. Microtubule dynamics in plant cells

    Henrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder and Clive W. Lloyd

  16. Drosophila S2 cells as a model system to investigate mitotic spindle dynamics, architecture and function

    Sara Moutinho-Pereira, Irina Matos and Helder Maiato

  17. Protein micropatterns: a direct printing protocol using deep UVs

    Ammar Azioune, Nicolas Carpi, Manuel Théry and Matthieu Piel

  18. Optical trapping and laser ablation of microtubules in fission yeast

    Nicola Maghelli and Iva M. Tolic-Nørrelykke

  19. Melanophores for Microtubule dynamics and motility assays

    Kazuho Ikeda, Irina Semenova, Olga Zhapparova, and Vladimir Rodionov

  20. New and old reagents for fluorescent-protein tagging of microtubules in fission yeast: experimental and critical evaluation

    Hilary A. Snaith, Andreas Anders, Itaru Samejima and Kenneth E. Sawin

  21. Imaging of mitotic spindle dynamics in C. elegans embryos

    Mika Toya, Yumi Iida and Asako Sugimoto

  22. A fast microfluidic temperature control device for studying microtubule dynamics in fission yeast

    Guilhem Velve-Casquillas, Judite Costa, Frédérique Carlier-Grynkorn , Adeline Mayeux and Phong T. Tran

  23. Microtubule-dependent spatial organization of mitochondria in fission yeast

    Maitreyi Das, Stephan Chiron and Fulvia Verde

  24. Photoactivatable GFP-Tubulin

    U. Serdar Tulu*, Nick P. Ferenz

  25. The Use of Fluorescence Redistribution After Photobleaching for Analysis of Cellular Microtubule Dynamics

    Claire E. Walczak, Rania S. Rizk and Sidney L. Shaw

  26. Analysis of microtubule polymerization dynamics in live cells

Sarah Gierke, Praveen Kumar, and Torsten Wittmann

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Cassimeris, Lynne.
Tran, Phong.

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