In this second of two new volumes covering mitochondria, methods developed to assess the number and function of nuclear-encoded proteins in the mitochondrion are presented. Chapters focus on the regulation of mitochondrial function and mitochondrial diseases, with a section emphasizing the mitochondrial defects associated with type 2 diabetes.
The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. With more than 450 volumes published, each volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences.
- New methods focusing on the examination of normal and abnormal mitochondrial function are presented in an easy-to-follow format by the researchers who developed them
- Along with a companion volume covering topics including mitochondrial electron transport chain complexes and reactive oxygen species, provides a comprehensive overview of modern techniques in the study of mitochondrial malfunction
- Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods
Defining the Mitochondrial Proteome 1. Method to define the mammalian mitochondrial proteome; V. Mootha 2. Proteome Characterization of Mouse Brain Mitochondria Using Electrospray Ionization Tandem Mass Spectrometry; X.Fang and C. S. Lee 3. Method to analyze tissue heterogeneity of the mammalian mitochondrial proteome; R. S. Balaban 4. Predicting proteomes of mitochondria and related organelles from genomic and expressed sequence tag data; D. Gaston, A. Tsausis and A. Roger 5. MitoP2: the mitochondrial proteome database; H. Prokisch Post-Translational Modifications of Mitochondrial Proteins 6. Reversible protein acetylation in mitochondria; E. Verdin 7.Post-translational modifications of rat liver mitochondria outer membrane proteins' C. L. Hoppel 8.Characterization of a novel ser/thr protein phosphatase in mitochondria; Y. Wang 9. R. Rizzuto 10.Selective enrichment in phosphopeptides for the identification of phosphorylated mitochondrial proteins; G. Pocsfalvi 11. Isolation of Regulatory-competent, Phosphorylated Cytochrome c and Cytochrome c Oxidase; M. Hüttemann 12. Identification of tyrosine phosphorylated proteins by Src kinases in rat brain; M. Salvi 13. Detection of a mitochondrial kinase complex that mediates an ERK-dependent phosphorylation of mitochondrial protein in steroid biosynthesis; E. J. Podestá 14. Dual specific phosphatases 18 and 21 target to opposing sides of the mitochondrial inner membrane; J. Rardin & J. E. Dixon 15.M. A. Kostiuk and B. O. Keller: "Isolation and identification of palmitoylated proteins in mitochondria using a chemical biology approach; L. G. Berthiaume Mitochondrial Dynamics 16.Mitochondrial fusion and movement using photoactivatable GFP; O. Shirihai 17. Regulation of mitochondrial fission and fusion by kinase and phosphatase activities. no title yet; S. Strack 18. Mitochondrial movement and synaptic function in Drosophila; T. L. Schwarz 19. The use of yeast and a novel biosensor to study mitochondrial ion transport, swelling, and mitophagy; R. J. Scheweyen 20.Complete Exchange of mtDNA's between Mouse Cells for Testing the Effects of mtDNA on Tumor Phenotypes; J. Hayashi Mitochondrial Function in Pancreatic Beta Cells 21. Coupling efficiency INS-1E cells; M. D. Brand and C. Affourtit 22. Methods in the study of substrate-specific differences on mitochondrial function mediating insulin secretion from pancreatic beta cells; B. E. Corkey 23. Knocking down of UCP 2 in animal models of obesity; L. A.Velloso 24.Roles of the cytosolic and mitochondrial forms of the malic enzyme on insulin secretion; G. W. Cline 25. Altered regulation of the PINK1 locus as a link between type 2 diabetes and neurodegeneration; J. A. Timmons 26. Insulin secretion from pancreatic beta cells is affected deletion of the nicotinamide nucleotide transhydrogenase; R. D. Cox 27. Determination of oxidative capacity and mitochondrial function in insulin resistance and type 2 diabetes; G. Shulman et al 28.Mitochondrial ATP production rate; K. S. Nair