Molecular Biology

  • ID: 1768338
  • Book
  • 280 Pages
  • Elsevier Science and Technology
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This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented.

Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.

  • Includes a glossary of over 200 terms important for understanding molecular biology
  • Uses an inexpensive source of eukaryotic cells - great for schools on a budget
  • Includes Methods Locator that provides instant access to the latest methods
  • Contain clearly written, easy-to-follow, student-tested instructions:
    • Sterile techniques
    • Phage titration
    • Gel electrophoresis of DNA
    • Restriction enzyme digestion
    • Plasmid isolation
    • Transformation of E. Coli
    • Recombinant DNA cloning
    • Nick translation labeling
    • Nonradioactive primer labelling
    • Nonradioactive DNA detection
    • Southern blotting
    • Colony hybridization
    • Purification of plant DNA
    • RNA purification
    • Northern blotting
    • Purification of poly A+ RNA
    • Polymerase chain reaction (PCR)
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Transposon Mutagenesis of Escherichia coliRecombinant DNA and Cloning
Southern Blot Analysis
Plant Genomic Southern Blotting with Probes for Low- and High-Copy-Number Genes
RNA Purification and Northern Blot Analysis
Polymerase Chain Reaction
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Karcher, Susan J.
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