- Provides most up-to-date information on techniques, sources, and equipment for anyone using molecular biological techniques
- Clearly presented information
- Divided into two parts which together comprise a complete collection of molecular biological methods
Nucleic Acid Blotting and Hybridization:
Membranes for Nucleic Acid Blotting.
Factors Influencing Hybridization.
Synthetic Oligonucleotide Probes.
Reagents and Conditions for Nucleic Acid Hybridization.
Chain Termination Sequencing.
Chemical Degradation Sequencing.
Denaturing Polyacrylamide Gel Electrophoresis.
DNA Databases and Software for Sequence Analysis.
Polymerase Chain Reaction:
Cloning PCR Products.
Sequencing PCR Products.
Labeling Nucleic Acids:
Detection of Radioactivity.
Protection from Radiochemical Hazards.
Agarose Gel Electrophoresis:
DNA and RNA Markers for Agarose Gels.
Calculation of the Relative Centrifugal Force.
Density Gradient Centrifugation.
Care of Rotors.
Chemicals and Reagents:
Details of Individual Compounds.
Protection from Chemical Hazards.
Protection from Microbiological Hazards.