Neoglycoconjugates are not only useful for the basic understanding of protein-carbohydrate interactions, but they have many practical applications as well. They are powerful reagents in many cell biology studies and excellent tools for the isolation and characterization of animal and plant lectins, separation of cells, as well as for the targeting of drugs, artificial vaccines, and diagnostic reagents. Volume 247 and its companion Volume 242 contain many practical methods on how to prepare and use neoglycoconjugates. Volume 242 deals with synthesis and 247 with biomedical applications.
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Enzymatic and Affinity Methods:M.M. Palcic, M. Pierce, and O. Hindsgaul, Synthetic Neoglycoconjugates in Glycosyltransferase Assay and Purification.A. Kobata, N. Kochibe, and T. Endo, Affinity Chromatography of Oligosaccharides on Psathyrella velutina Lectin Column.S. Sabesan, J.C. Paulson, and J. Weinstein, Separation of Galb1, 4G1cNAc a-2,6- and Galb1,3(4)GlcNAc a-2,3-Sialyltransferases by Affinity Chromatography.E. Altman and D.R. Bundle, Polysaccharide Affinity Columns for Purification of Lipopolysaccharide-Specific Murine Monoclonal Antibodies.M.-C. Shao and C.Q.C. Chin, Streptavidin-Biotinylglycopeptide-Lectin Complex in Detection of Glycopeptides and Determination of Lectin Specificity.
Binding Site Characterization:J. Lehmann and M. Schmidt-Schuchardt, Spacer-Modified Oligosaccharides as Photoaffinity Probes for Porcine Pancreatic (-Amylase.D.R. Bundle and B.W. Sigurskjold, Determination of Accurate Thermodynamics of Binding by Titration Microcalorimetry.C.P.J. Glaudemans, P. Kovac, and E.M. Nashed, Mapping of Hydrogen Bonding between Saccharides and Proteins in Solution.
Biomedical Applications:T. Toyokuni and S.-I. Hakomori, Carbohydrate(Lysyllysine Conjugates as Cell Antiadhesion Agents.M.A. Findeis, C.H. Wu, and G.Y. Wu, Ligand-Based Carrier Systems for Delivery of DNA to Hepatocytes.R. Roy, C.A. Laferriore, R.A. Pon, and A. Gamian, Introduction of Rabbit Immunoglobulin G Antibodies against Synthetic Sialylated Neoglycoproteins.N. Sugiura and K. Kimata, Syntheses and Functions of Neoproteoglycans: Lipid-Derivatized Chondroitin Sulfate with Antiadhesion Activity.M. Kudo, D.R. Vera, and R.C. Stadalnik, In Vivo Quantification of Asialoglycoprotein Receptor.M. Kudo, K. Washino, Y. Yamamichi, and K. Ikekubo, Synthesis and Radiolabeling of Galactosyl Human Serum Albumin.D.R. Vera, S.J. Topcu, and R.C. Stadalnik, In Vitro Quantification of Asialoglycoprotein Receptor Density from Human Hepatic Microsamples.D.R. Vera, R.C. Stadalnik, M. Kudo, and K.A. Krohn, Radiopharmaceutical Preparation of Technetium-99m-Labeled Galactosyl-Neoglycoalbumin.K. Kobayashi, A. Kobayashi, and T. Akaike, Culturing Hepatocytes on Lactose-Carrying Polystyrene Layer via Asialoglycoprotein Receptor-Mediated Interactions.