Nitric Oxide, Part G, Vol 441. Methods in Enzymology

  • ID: 1768793
  • Book
  • 488 Pages
  • Elsevier Science and Technology
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The Nobel Prize was awarded in Physiology or Medicine in 1998 to Louis J. Ignarro, Robert F. Furchgott and Ferid Murad for demonstrating the signaling properties of nitric oxide. Nitric oxide (NO) is one of the few gaseous signaling molecules and is a key biological messenger that plays a role in many biological processes. NO research has led to new treatments for treating heart as well as lung diseases, shock and impotence. (Sildenafil, popularly known by the trade name Viagra, enhances signaling through NO pathways.) Scientists are currently testing whether NO can be used to stop the growth of cancerous tumors, since the gas can induce programmed cell death, apoptosis.

This is another "must-have” volume packed with robust methods from authors around the globe. Researchers interested in the detailed biochemistry of NO and its synthesis will have this indispensable volume on their shelves.

*Essential resource for every laboratory involved in NO-related work

*Gathers tried and tested techniques from global labs which eliminates searching through many different sources and avoids pitfalls so the same mistakes are not made over and over.

* Aids researchers in the design of medically important therapies for heart disease and cancer
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Chapter One: Protein 3-Nitrotyrosine in Complex Biological Samples: Quantification by High-Pressure Liquid Chromatography/Electrochemical Detection and Emergence of Proteomic Approaches for Unbiased Identification of Modification Sites

Chapter Two: Selective Fluorogenic Derivatization of 3-Nitrotyrosine and 3,4-Dihydroxyphenylalanine in Peptides: A Method Designed for Quantitative Proteomic Analysis

Chapter Three: Nitroalkenes: Synthesis, Characterization, and Effects on Macrophage Activation

Chapter Four: In-Gel Detection of S-Nitrosated Proteins Using Fluorescence Methods

Chapter Five: The Arachidonate-Dependent Survival Signaling Preventing Toxicity in Monocytes/Macrophages Exposed to Peroxynitrite

Chapter Six: Practical Approaches to Investigate Redox Regulation of Heat Shock Protein Expression and Intracellular Glutathione Redox State

Chapter Seven: Monitoring Oxidative Stress in Vascular Endothelial Cells in Response to Fluid Shear Stress: From Biochemical Analyses to Micro- and Nanotechnologies

Chapter Eight: Determination of S-Nitrosothiols in Biological and Clinical Samples Using Electron Paramagnetic Resonance Spectrometry with Spin Trapping

Chapter Nine: Novel Method for Measuring S-Nitrosothiols Using Hydrogen Sulfide

Chapter Ten: Kinetic Studies on Peroxynitrite Reduction by Peroxiredoxins

Chapter Eleven: Nitrocytochrome c: Synthesis, Purification, and Functional Studies

Chapter Twelve: Tyrosine Nitration, Dimerization, and Hydroxylation by Peroxynitrite in Membranes as Studied by the Hydrophobic Probe N-T-BOC-L-tyrosine tert-Butyl Ester

Chapter Thirteen: Assessment of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation Products

Chapter Fourteen: Methods to Measure the Reactivity of Peroxynitrite-Derived Oxidants Toward Reduced Fluoresceins and Rhodamines

Chapter Fifteen: Detection and Characterization of Peroxynitrite-Induced Modifications of Tyrosine, Tryptophan, and Methionine Residues by Tandem Mass Spectrometry

Chapter Sixteen: Reductive Gas-Phase Chemiluminescence and Flow Injection Analysis for Measurement of the Nitric Oxide Pool in Biological Matrices

Chapter Seventeen: Detection and Measurement for the Modification and Inactivation of Caspase by Nitrosative Stress In Vitro and In Vivo

Chapter Eighteen: Interactive Relations between Nitric Oxide (NO) and Carbon Monoxide (CO): Heme Oxygenase-1/CO Pathway Is a Key Modulator in NO-Mediated Antiapoptosis and Anti-inflammation

Chapter Nineteen: Detection and Characterization of In Vivo Nitration and Oxidation of Tryptophan Residues in Proteins

Chapter Twenty: In Vivo Real-Time Measurement of Nitric Oxide in Anesthetized Rat Brain

Chapter Twenty-One: Nitric Oxide and Cardiobiology-Methods for Intact Hearts and Isolated Myocytes

Chapter Twenty-Two: Microscopic Technique for the Detection of Nitric Oxide-Dependent Angiogenesis in an Animal Model

Author Index

Subject Index

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Cadenas, Enrique.
Packer, Lester.

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