Organized into 15 chapters, the book covers methods for the study of virtually every major group of entomopathogen, as well as methods for discovery and diagnosis of entomopathogens and the use of complementary methods for microscopy. It discusses the use of molecular techniques for identifying and determining phylogeny, factors that contribute to resistance to entomopathogens, and several other aspects of the science of invertebrate pathology. It also explains initial handling and diagnosis of diseased invertebrates, basic techniques in insect virology, and bioassay of bacterial entomopathogens against insect larvae. In addition, the reader is introduced to the use of bacteria against soil-inhabiting insects and preservation of entomopathogenic fungal cultures. The remaining chapters focus on research methods for entomopathogenic microsporidia and other protists, how the pathogenicity and infectivity of entomopathogens to mammals are tested, and preparations of entomopathogens and diseased specimens for more detailed study using microscopy.
Experienced insect pathologists, biologists, entomologists, students, biotechnology personnel, technicians, those working in the biopesticide industry, and government regulators will find this manual extremely helpful.
- Step-by-step instructions for the latest techniques on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens- New edition fully updated to address changes in the taxonomy of the vast majority of taxa - Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques - Provides extensive supplemental literature and recipes for media, fixatives and stains
Please Note: This is an On Demand product, delivery may take up to 11 working days after payment has been received.
Manual of Techniques in Invertebrate Pathology, 2nd edition
Lawrence A. Lacey, editor
1. Initial Handling and Diagnosis of Diseased Invertebrates
Lawrence A. Lacey and Leellen Solter
2. Basic Techniques in Insect Virology
Karolin E. Eberle, Jörg T. Wennmann, Regina G. Kleespiesand Johannes A. Jehle
3. Isolation, Culture, Preservation, and Identification of Entomopathogenic Bacteria of the Bacilli.
Tanja W. Fisher and Steven F. Garczynski
4. Bioassay of Bacterial Entomopathogens Against Insect Larvae
Maureen O'Callaghan, Travis R. Glare and Lawrence A. Lacey
5. Bacteria for use Against Soil-Inhabitating Insects
Albrecht M. Koppenhöfer, Trevor A. Jackson, and Michael G. Klein
6. Identification of Entomopathogenic Fungi
Richard A. Humber
7. Laboratory Techniques Used for Entomopathogenic Fungi: Hypocreales
G. Douglas Inglis, Juerg Enkerli, and Mark S. Goettel
8. Mass Production of Entomopathogenic Hypocreales
Stefan T. Jaronski and Mark A. Jackson
9. Methods for the Study of Entomophthorales
Ann E. Hajek, Bernard Papierok, and Jørgen Eilenberg
10. Preservation of Entomopathogenic Fungal Cultures
Richard A. Humber
11. Research Methods for Entomopathogenic Microsporidia and Other Protists
Leellen Solter, James Becnel, and Jiri Vávra
12. Nematode Parasites, Pathogens and Associates of Insects and Invertebrates of Economic Importance
S. Patricia Stock and Heidi Goodrich-Blair
13. Pathogens and Parasites of Terrestrial Molluscs
Michael J. Wilson
14. Testing the Pathogenecity and Infectivity of Entomopathogens to Mammals
Joel P. Siegel
15. Complementary Techniques: Preparations of Entomopathogens and Diseased Specimens for More Detailed Study Using Microscopy
James J. Becnel
Dr. Lacey earned his PhD in 1978 and has been internationally active working in medical entomology, insect pathology, and microbial control of economically and medically important insects. He conducted research on the biology and microbial control of dozens of insects in the US, Amazon Basin, West Africa in the Azores, throughout Europe, the Middle East and Western and Southeast Asia and the Pacific Northwest of the USA. In his 40 year career he has written or co-authored nearly 150 refereed papers in medical entomology, agricultural entomology, and insect pathology, and edited and co-edited seven books, including the Manual of Techniques in Invertebrate Pathology, second edition and Microbial Control of Insect and Mite Pests: From Theory to Practice.