Everything you need to begin conducting your own proteomics research
Proteomics: Introduction to Methods and Applications is the ideal introduction to the field of proteomics, giving readers the skills and knowledge needed to study proteins, their structure, and their function in cells, tissues, and organisms. Written by experienced researchers, the text not only covers all the basic concepts, techniques, and applications, it also offers readers insights and practical tips based on the authors′ many years of hands–on work in proteomics research. Moreover, the text has been carefully edited to ensure a thorough, consistent, and highly readable approach throughout all the chapters.
This text offers everything readers need to begin conducting their own proteomics research, including:
A full chapter dedicated to laboratory exercises and protocols to help readers apply their skills
Step–by–step explanations of key analytical methods, including mass spectrometry, sample preparation, chromatographic and electrophoretic separation, and micro–total analysis systems
Complete coverage of bioinformatics, including an introduction to important proteomics databases
A glossary defining the key terms in the field
An appendix with an annotated list of Internet resources, information on post–translational modifications, and the masses and compositions of amino acid residues
This text is designed for upper–level undergraduate and graduate courses in proteomics. Upon successful completion of the text, students are able to apply their skills and knowledge to solve problems in such fields as biotechnology, drug discovery, and medical research.
1. Introduction to Proteomics and Strategy for Proteins Identification.
2. Sample Preparation.
3. Chromatographic Separation Methods.
3.1. Liquid Chromatography Linked to Mass Spectrometry.
3.2. Multidimensional Liquid Chromatography Combined with Mass Spectrometry.
4. Electrophoretic Separation Methods.
4.1. One–Dimensional Gel Electrophoresis.
4.2. Two–Dimensional Gel Electrophoresis.
5. Capillary Electrophoresis.
6. Protein Blotting.
7. Introduction to Proteins and Peptides Mass Spectrometry.
7.1. Electrospray Ionization (ESI).
7.2. Matrix–Assisted Laser Desorption Ionization (MALDI).
7.3. Imaging Mass Spectrometry.
7.4. Fragmentation and Sequencing in Proteomics.
7.5. Protein Identification by Mass Spectrometry.
7.6. Chemical Derivatization for Sequence Analysis.
8. Protein Modifications.
10.1. Quantitative Proteomics.
10.2. Structural Proteomics.
10.3. Functional Proteomics.
10.4. Clinical Proteomics.
13. The Laboratory Practicals.
13.1. Polyacrylamide Gel Electrophoresis under Denaturing Conditions.
13.2. Preparation of the In–Gel Separated Proteins for Mass Spectrometry.
13.3. Bioinformatic Tools for Protein Identification.
13.4. LC–MS and LC–MS/MS – Proteomics Applications.
13.5. Enzyme Kinetics.
13.6. Internet Databases.