The first resource of its kind, Human Stem Cell Technology and Biology presents laboratory techniques and protocols that have proven to be effective for the culture of pluripotent stem cells, as well as current perspectives in the field. Edited by a team of professionals at the forefront of discovery and innovation in stem cell research, education, and development of infrastructure, this authoritative multimedia training manual distinguishes itself by integrating readily accessible text, electronic, and video components to explain the biology, technology, and applications of human embryonic stem cells.
The first section of the text begins with an overview of the research that established the foundation for current initiatives in stem cell research; it goes on to describe the best methods for researching and obtaining pluripotent cell lines. The second section provides the skills and techniques necessary for the culture, maintenance, and characterization of pluripotent stem cells. This section has been developed as a multimedia stem cell course in order to present the most comprehensive instruction possible. The third section presents an in–depth perspective on the current status of the stem cell research field, and the fourth section explores applications, describing the most recent advances in research and the interface with gene therapy, tissue engineering, and drug testing.
Features of this one–of–a–kind reference include:
Introductory chapters that provide background information to scientists entering the field
State–of–the–art, contemporary perspectives written by international leaders in the field
Videos demonstrating step–by–step laboratory procedures for the culture of human embryonic and induced pluripotent stem cells on the accompanying DVD
Printable laboratory protocols in PDF form, accessible on the book′s Web site
Online updates of the rapidly developing strategies and research protocols that support stem cell research
Human Stem Cell Technology and Biology is an invaluable reference and laboratory manual for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field. Whether it is used to design a laboratory curriculum, workshop, or training course, or simply to assist in performing research in stem cell biology, this volume is a must–have resource of protocols, information, and perspectives for professional laboratories, colleges, universities, and vocational schools.
Editors and Contributors.
SECTION I: INTRODUCTION.
1 Introduction to Pluripotent Stem Cells: Biology and Applications (Maria Borowski and Gary S. Stein).
2 Researching and Obtaining Established Stem Cell Lines (Mai X. Luong, Kelly P. Smith, and Gary S. Stein).
SECTION II: LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE: PLURIPOTENT STEM CELL CULTURE.
3 Basics of Cell Culture (Alicia Allaire, Mai X. Luong, and Kelly P. Smith).
4 The Stem Cell Laboratory (Alicia Allaire, Mai X. Luong, and Kelly P. Smith).
5 Reagent Preparation (Alicia Allaire, Mai X. Luong, and Kelly P. Smith).
6 Preparation of Mouse Embryonic Fibroblasts for Culture of Human Embryonic Stem Cells (Meng–Jiao Shi, Maria Borowski, and Kimberly Stencel).
7 Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts (Meng–Jiao Shi, Maria Borowski, and Kimberly Stencel).
8 Thawing, Seeding, and Changing the Medium of Human Embryonic Stem Cells (Meng–Jiao Shi, Kimberly Stencel, and Maria Borowski).
9 Passaging of Human Embryonic Stem Cells on Inactivated Mouse Embryonic Fibroblast Plates (Meng–Jiao Shi, Kimberly Stencel, and Maria Borowski).
10 Harvesting Human Embryonic Stem Cells for Cryopreservation (Meng–Jiao Shi, Kimberly Stencel, and Maria Borowski).
11 Human Embryonic Stem Cell Culture on BD Matrigel with mTeSR®1 Medium (Meng–Jiao Shi, Kimberly Stencel, and Maria Borowski).
SECTION III: LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE: CHARACTERIZATION OF PLURIPOTENT STEM CELLS.
12 Defining Pluripotency (Kelly P. Smith and Mai X. Luong).
13 Characterization of Human Embryonic Stem Cells by Immunofluorescence Microscopy (Shirwin M. Pockwinse and Prachi N. Ghule).
14 Preparation of Human Embryonic Stem Cell Samples for Flow Cytometry (Meng–Jiao Shi, Kimberly Stencel, and Maria Borowski).
15 Characterization of Human Embryonic Stem Cell Gene Expression Using Reverse Transcription Polymerase Chain Reaction (Alicia Allaire, Kelly P. Smith, and Mai X. Luong).
16 Determining Pluripotency of Human Embryonic Stem Cells: Embryoid Body Formation (Shirwin M. Pockwinse and Prachi N. Ghule).
17 Characterization of Human Embryonic Stem Cells by Cytogenetics: Karyotyping and Fluorescence In Situ Hybridization (Shirwin M. Pockwinse, Prachi N. Ghule and Anne Higgins).
18 High–Resolution Chromatin Immunoprecipitation Assay (Beatriz Perez–Cadah a, Bojan Drobic, and James R. Davie).
19 Assaying Pluripotency Via Teratoma Formation (M. William Lensch and Tan A. Ince).
SECTION IV: PERSPECTIVES IN HUMAN STEM CELL TECHNOLOGIES.
20 Genomic Analysis of Pluripotent Stem Cells (David Lapointe).
21 Proteomic Analysis of Human Pluripotent Cells (Andy T. Y. Lau, Yan–Ming Xu, and Jen–Fu Chiu).
22 Bioinformatics Strategies for Understanding Gene Expression in Human Pluripotent Cells (Gustavo Glusman, Bruz Marzolf, Kai Wang, Ji–Hoon Cho, Burak Kutlu, and Qiang Tian).
23 Epigenetic Analysis of Pluripotent Cells (Mojgan Rastegar, Genevieve P. Delcuve, and James R. Davie).
24 Differentiation of Pluripotent Stem Cells: An Overview (Jeremy Micah Crook).
25 Cellular Reprogramming: Current Technology, Perspectives, and Generation of Induced Pluripotent Cells (Tanja Dominko).
SECTION V: APPLICATIONS OF HUMAN EMBRYONIC STEM CELLS.
26 Human Pluripotent Cells: The Biology of Pluripotency (Li–Fang Chu and Thomas P. Zwaka).
27 Human Pluripotent Cells for Regenerative Medicine: Potential Applications for Regenerative Medicine (Christopher C. Ford and Darrell N. Kotton).
28 Therapeutic Applications of Human Embryonic Stem Cells (Shi–Jiang Lu, Irina Klimanskaya, Edmund Mickunas, and Robert Lanza).
29 Human Stem Cells for Drug Screening, Toxicity, Specificity, and Off–Target Effects (Arnaud Lacoste and Mark Burcin).
30 Interfacing Stem Cells with Gene Therapy (Christian Mueller and Terence R. Flotte).
31 Tissue Engineering for Stem Cell Mediated Regenerative Medicine (Janet Zoldan, Thomas P. Kraehenbuehl, Abigail K. R. Lytton–Jean, Robert S. Langer, and Daniel G. Anderson).