An invaluable introduction to the basic concepts of the subject, this text offers the student an excellent overview of current research methods and applications and is a good starting point for those new to the area.
- A clear, concise introduction to the subject of modern genomic analysis
- A technology-oriented approach including the latest developments in the field
- Invaluable to those students taking courses in Bioinformatics, Human Genetics, Biochemistry and Molecular Biology
1 General introduction.
1.1 Revision of molecular genetics.
1.2 The tools of molecular biology.
1.3 Specifics of the genome programmes.
1.4 The species analysed.
2 Linkage maps.
2.1. Tools and methods in genetic mapping.
2.2 The development of genetic maps.
2.3 Radiation hybrid maps.
3 Physical maps.
3.1 Local maps or small genomes.
3.2 Strategies for physical mapping of the human genome.
3.3 Maps of the human genome.
4 Genome sequencing .
4.1 Strategic choices.
4.2 Prokaryotic genomes.
4.3 Genomes of model eukaryotes.
4.4 The human genome. & lt;div>
5 Sequencing cDNA and the transcriptome.
5.1 Strategies of cDNA sequencing.
5.2 The economic stakes.
5.3 The analysis of cDNA sequences.
5.4 The transcriptome.
6 The proteome.
6.1 Basic techniques.
6.2 Transgenesis .
6.4 Two-dimensional electrophoresis and identification of proteins.
6.5 Identification of protein interactions by twin hybrids.
6.6 Chip technology.
6.7 Analysis of three-dimensional structure.
7 Identification of genes responsible for disease.
7.1 Genetic diseases.
7.2 Functional cloning and chromosomal anomalies.
7.3 Strategy for positional cloning.
7.4 What is the future for the cloning of disease-causing genes?