Primary and Stem Cells. Gene Transfer Technologies and Applications

  • ID: 2171446
  • Book
  • 296 Pages
  • John Wiley and Sons Ltd
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The all–important resource that lays the groundwork for researchers to genetically modify primary and stem cells for use in downstream applications

Replacing damaged or lost organs by producing "spare parts" is a concept that lies at the core of the varied biotechnological practices generally referred to as tissue engineering. An important component in this regeneration process includes the use of stem cells, which harbor the ability to mature into other cell types and hold the potential to act as a repair system for the body. Primary and Stem Cells describes basic cell engineering methods, emphasizing stem cell applications, and the use of genetically modified stem cells in cell therapy and drug discovery. Discussion includes new techniques for the engineering of stem cells and the delivery of transgenes into stem cells via various viral and non–viral systems, along with:

  • Transfection techniques and methods for cell line engineering

  • Information that assists researchers and drug developers in deciding how to generate their required drug or gene therapists in developing genetically modified cells

  • An overview of applications in areas like disease models and drug discovery

  • Issues that address the interests of companies involved in stem cell tools and reagents

Primary and Stem Cells serves as an essential guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator′s purpose basic science investigation, creation of disease models and screens, or cells for therapeutic applications and acts as the launching pad for pioneering further development in a field emerging with exciting possibilities.

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FOREWORD viiMichele Calos




1. DNA Assembly Technologies Based on Homologous Recombination 3Billyana Tsvetanova, Lansha Peng, Xiquan Liang, Ke Li, Jian–Ping Yang, Tony Ho, Josh Shirley, Liewei Xu, Jason Potter, Wieslaw Kudlicki, Todd Peterson, and Federico Katzen

2. Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized All–IN–One Expression Construct for Generating Mouse iPS Cells 19Takefumi Sone, Yoko Takata, Hiroe Kishine, Taichi Andoh, Fumio Imamoto, and Jonathan D. Chesnut

3. Strategies for the Delivery of Naked DNA 37Jennifer C. Moore and Ronald P. Hart


4. Episomal Vectors 51
Junying Yu

5. Nonintegrating DNA Virus 85Chris Kemp and David Kuninger

6. Nonintegrating RNA Viruses 103Noemi Fusaki

7. Protein Delivery 119Xiao–Jian Han and Kazuhito Tomizawa


8. Sleeping Beauty Transposon–Mediated Stable Gene Delivery 137Andrew Wilber, Xianzheng Zhou, Xin Huang, Dan S. Kaufman, and R. Scott McIvor

9. Integrating Viral Vectors for Gene Modifications 163Gemma L. Mendel, Brian Paszkiet, and Rafal P. Witek

10. Bacteriophage Integrases for Site–Specific Integration 199Bhaskar Thyagarajan

11. Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells 211Sangyoon Han, Anna McCann, Louise C. Laurent, Jeanne F. Loring, and Ying Liu


12. Modified Stem Cells as Disease Models and in Toxicology Screening 229
Catharina Ellerstr om, Peter Sartipy, Raimund Strehl, and Johan Hyllner

13. Screening and Drug Discovery 251Elizabeth A. Davenport and Robert S. Ames


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This is an excellent resource on the new techniques for the engineering of stem cells and the delivery of transgenes into stem cells via various viral and nonviral systems. It provides comprehensive information on the concepts of cell engineering principles and their application in primary and stem cells.  Researchers, established stem cell scientists, and graduate students interested in this field will find this book very handy.   (Doody s, 17 August 2012)

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