Playing a prominent role as a signal transducer, calcium is an important participant in the communication pipeline directing cellular activity. This expert guide explains the unique and highly diverse functions of calcium in biology, culminating with a discussion of the calcium binding proteins that act as secondary messengers in cells and are responsible in controlling vital bodily mechanisms such as muscle contraction and the regulation of enzymes.
A comprehensive volume reflecting breakthrough science, Calcium Binding Proteins:
Contains current information on all major families of calcium binding proteins including their structural, physico–chemical and functional properties, as well as their evolution
Discusses techniques that underlie the description of proteins, including NMR, circular dichroism, optical rotatory dispersion spectroscopy, calorimetry, and crystallography
Describes the structures, physical characteristics, functions, and general patterns in the evolution of calcium binding proteins
This book relies on the most current biological findings to cover the patterns of biochemical phenomena such as calcium homeostasis, min–eralization, and cell signaling involving specific proteins. Summarizing ongoing studies and presenting general hypotheses that help to focus future research, Calcium Binding Proteins provides newcomers to this field as well as established professionals with a conceptual framework instru–mental in advancing their scientific pursuits.
1. Historical Perspective.
2. Physiological Processes Involving Calcium Binding Proteins.
3. Comparison of the Ca2+ Ion with Other Metal Cations.
4. Complexes of Calcium and Other Cations with Low Molecular Weight.
5. Stoichiometry, Kinetics, and Thermodynamics of Calcium Binding.
6. Experimental Methods Used to Study Calcium Binding to Proteins.
7. Structure and Evolution of Proteins.
8. Protein Complexes with Metals Other than Calcium.
9. Nonessential Metals.
11. EF–Hand Proteins.
12. Cytosolic Calcium Binding Proteins Lacking EF–Hands.
13. Extracellular Calcium Binding Proteins.
14. Interactions of Calcium Binding Proteins with Other Metal Ions.
15. Interactions of Calcium Binding Proteins with Other Proteins and with Membranes.
16. Genetic Engineering of Calcium Binding Proteins.
Robert H. Kretsinger received his PhD in biophysics at the Massachusetts Institute of Technology under the supervision of Alex Rich in 1964. In 1967 he set up a protein crystallography lab in the Department of Biology at the University of Virginia, where he¿presently teaches, and determined the crystal structure of parvalbumin as well as describing the EF–hand domain in 1971. He has subsequently investigated the structures, functions, and evolution of various EF–hand homologs, along with other protein families. From 1978 to 1995, he directed a national facility to build multiwire area detectors for x–ray diffraction and to collect data from protein crystals.