The introductory section provides an overview of vaccine research and looks at how genomics can complement traditional approaches. The second section focuses on advanced technologies, such as genome sequence analysis, microarrays, proteomics and high–throughput cloning.
The third section constitutes the unique part of this book, with six chapters that describe the application of genomic and proteomic techniques in the search for new antibacterial vaccines. The target pathogens include meningococcus B, streptococci, chlamydia and Pseudomonas.
Infectious diseases are once again threatening the health of human populations, even in countries where they were thought to have been controlled. With the spread of antibiotic resistance, the concept of prevention rather than cure becomes ever more important. The new genomic approaches described in this book will help to accelerate vaccine development and benefit the health of millions of people worldwide.
This book should be of interest to all those working in vaccine discovery and development in pharmaceutical and biotechnology companies as well as in academic institutions.
List of Contributors.
PART 1: INTRODUCTION.
1. Vaccination: Past, Present and Future (Maria Lattanzi and Rino Rappuoli).
2. Bioinformatics, DNA Microarrays and Proteomics in Vaccine Discovery: Competing or Complementary Technologies? (Guido Grandi).
PART 2: TECHNOLOGIES.
3. Genome Sequencing and Analysis (Hervé Tettelin and Tamara Feldblyum).
4. Understanding DNA Microarrays: Sources and Magnitudes of Variances in DNA Microarray Data Sets (She–pin Hung, G. Wesley Hatfield, Suman Sundaresh and Pierre F. Baldi).
5. The proteome, Anno Domini Two Zero Zero Three (Pier Giorgio Righetti, Mahmoud Hamdan, Frederic Reymond and Joël S. Rossier).
6. Mass Spectrometry in Proteomics (Pierre–Alain Binz).
7. High Throughput Cloning, Expression and Purification Technologies (Andreas Kreusch and Scott A. Lesley).
PART 3: APPLICATIONS.
8. Meningococcus B: from Genome to Vaccine (Davide Serruto, Rino Rappuoli and Mariagrazia Pizza).
9. Vaccines Against Pathogenic Streptococci (John L. Telford, Immaculada Margarit y Ros, Domenico Maione, Vega Masignani, Hervé Tettelin, Giuliano Bensi and Guido Grandi).
10. Identification of the ′Antigenome′ a Novel Tools for Design and Development of Subunit Vaccines Against Bacterial Pathogens (Eszter Nagy, Tamás Henics, Alexander von Gabain and Andreas Meinke).
11. Searching the Chlamydia Genomes for New Vaccine Candidates (Giulio Ratti, Oretta Finco and Guido Grandi).
12. Proteomics and Anti–Chlamydia Vaccine Discovery (Gunna Christiansen, Svend Birkelund, Brian B. Vandahl and Allan C Shaw).
13. Proteome Analysis of Other Membrane and Extracelular Proteins from Pseudomonas aeruginosa for vaccine Discovery (Stuart J. Cordwell and Amanda S. Nouwens).