The book′s authors begin each chapter with an overview of the important biological questions presently being considered in their field, then present scientific approaches to address these questions. In explaining approaches, the authors cover experimental approaches, conceptual frameworks, data obtained, and pitfalls common to the techniques. Easy–to–reference protocol sections offer flow diagrams and illustrations to better elucidate new and unfamiliar techniques. Coverage in Transmembrane Transporters includes:
∗ Expression strategies
∗ High–throughput screening
∗ Molecular genetics
∗ Structural analyses, such as NMR, crystallization, freeze fracture, and chimeras
∗ Functional analyses, such as imaging, electrophysiology, and microdialysis
Transmembrane Transporters provides readers new to the field with an accessible introduction to the wide variety of transporter research approaches, while offering practicing researchers a thorough analysis of the cutting–edge work currently being performed. This text proves a compelling resource for students and professionals in neuroscience, molecular biology, biochemistry, and biophysics.
Families of Transporters and Their Classification (M. Saier).
Yeast as a Model System for Studying Glucose Transport (E. Boles).
Neurotransmitter Transporters of Drosophila (M. Burg & W. Pak).
Transgenic Mice in Monoamine Transporter Research (S. Jones).
Searching for Novel Genetic Variation in Neurotransmitter Transporters (R. Blakely & A. Belous).
Nonviral Gene Transfer Allows Up– and Down–Expression of the Brain Serotonin Transporter with Functional Consequences (M. Martres, et al.).
Methods in Studying the Regulation and Trafficking of Transmembrane Transporters (S. Deken, et al.).
Chemical Modification Strategies for Structure–Function Studies (G. Rudnick).
Methanethiosulfonate Reagent Accessibility Studies, Cysteine–Scanning Mutagenesis, Protein Overexpression, and Functional Reconstitution: A Strategy for Studying the Structure/Function Relationships within the Mitochondrial Citrate Transport Protein (R. Kaplan).
Peptide Mapping of Dopamine Transporter Ligand and Substrate Interaction Sites (M. Lowe, et al.).
Mass Spectrometry of Membrane Transport Proteins (J. Whitelegge, et al.).
Amperometric Recording of Amphetamine–Induced Dopamine Efflux (D. Sulzer, et al.).
Voltage Clamp and Fluorometric Techniques for Studying Glutamate Transporter Function (A. Tzingounis, et al.).
Studies of Glial Glutamate Transporters in Hippocampal Microcultures (S. Mennerick, et al.).
Imaging Monoamine Transporters in the Brain (H. Kung & M. Kung).
Measuring and Modeling the Spatiotemporal Profile of GABA at the Synapse (L. Overstreet, et al.).
...introduces many technologies to be used in future studies....people, who are interested in transporter research, can get new ideas for next research from this book. (Pharmaceutical Research, Vol. 20, No. 12, December 2003)