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Chemical Measurements in Biological Systems. Edition No. 1. Techniques in Analytical Chemistry

  • ID: 2172426
  • Book
  • June 2000
  • 280 Pages
  • John Wiley and Sons Ltd
Techniques in Analytical Chemistry
How to evaluate and validate data from chemical measurements in biological systems-a unique overview
An understanding of chemical measurement processes in biological systems is essential to working in today's life-science and biotechnology industries. This work provides analysts and laboratory professionals with the fundamental knowledge and basic principles they need to tackle the measurement process in the real world. Rather than rehashing traditional analytical techniques, which can be problematic for biological samples, the book emphasizes the highly complex nature of biological systems through such topics as enzyme measurements, enzymes as reagents, immunochemical assays, and assay validation and quality control. It provides a complete overview of principles and methods proven useful in biological systems-from pH, buffers, and spectrophotometry to basic chromatographic techniques. With Chemical Measurements in Biological Systems as their guide, readers will understand the basic principles needed to:

Read and evaluate an assay method description

Choose the appropriate method from the vast array of available techniques

Modify and evaluate an existing method for specific needs

Set up and run biochemical assays

Use multistep problem-solving techniques

Evaluate the results of a given assay in a given matrix
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Water, pH, and Buffers.

Ultraviolet and Visible Absorption Spectrophotometry and Photometry.

Detection Reactions (Colorimetric Reactions).

Enzymes and the Use of Enzymes as Reagents.

Measurement of Enzyme Activities.

Chromatography as a Tool for Measurement of Analytes in Complex Mixtures.

Electrophoresis and Other Electrokinetic Separations.

Enzyme-Linked Immunosorbant Assays.

Assay Quality Control and Data Validation.

Method Selection.

Appendices.

Index.
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Kent K. Stewart
Richard E. Ebel
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