Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state–of–the–art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high–speed sorting and alternative sorting methods, as well as traditional technology. This essential resource:
∗ Assumes no prior knowledge of flow cytometry
∗ Progresses with an informal, engaging lecture style from simpleto more complex concepts
∗ Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis
∗ Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields
Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.
Acknowledgments (First Edition).
Acknowledgments (Second Edition).
The Past as Prologue.
Setting the Scene.
Instrumentation: Into the Black Box.
Information: Harnessing the Data.
Seeing the Light: Lasers, Fluorochromes, and Filters.
Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating.
Cells from Within: Intracellular Proteins.
Cells from Within: DNA in Life and Death.
The Sorting of Cells.
Disease and Diagnosis: The Clinical Laboratory.
Out of the Mainstream: Research Frontiers.
Flowing On: The Future.