Current Protocols Essential Laboratory Techniques. 2nd Edition

  • ID: 2174355
  • Book
  • 664 Pages
  • John Wiley and Sons Ltd
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Provides students with the solid grounding in biological laboratory techniques needed to pursue any major in the life and health sciences

Building on the success of its American Publishers Award–winning predecessor, Current Protocols Essential Laboratory Techniques, Second Edition gives new researchers the skills and understanding of the fundamental laboratory procedures that are needed to run experiments, solve problems, and become a productive member of the modern life science laboratory.

An indispensable tool for readers, this Second Edition includes chapters on cell culture techniques, electrophoresis, microscopy, enzymatic reactions, and bioinformatics. In addition to covering measurement, preparation of reagents, blotting, chromatography, and real–time PCR, Current Protocols Essential Laboratory Techniques also:

  • Teaches new investigators how to perform basic laboratory research techniques

  • Explains solution chemistry and preparation

  • Describes care and use of common equipment such as pH meters, spectrophotometers, centrifuges, and microscopes

  • Covers basic laboratory safety

  • Provides access to Current Protocols Essential Laboratory Techniques online

Written by distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, Second Edition is a great source for beginning scientists who want to develop the skills for a fruitful career in research.

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Foreword

Preface 

Contributors List

Conversion Factors

Flow Chart: Application of TechniquesEmily Wiley, Claremont Colleges

Chapter 1 Volume/Weight Measurement

Unit 1.1 Volume Measurement
Andrew Zanella and Tom Davis, Claremont Colleges

Unit 1.2 Weight Measurement
Michael Guzy, Ohaus, Corp.

Chapter 2 Concentration Measurement

Unit 2.1 Spectrophotometry
Rob Morris, Ocean Optics, Inc.

Unit 2.2 Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Sean R. Gallagher, UVP, LLC

Chapter 3 Reagent Preparation

Unit 3.1 Theoretical Discussion
Deb Chakravarti, Keck Graduate Institute

Unit 3.2 Practical Discussion
Deb Chakravarti, Keck Graduate Institute

Unit 3.3 Recipes

In–House

Chapter 4 Cell Culture Techniques

Unit 4.1 Aseptic Technique
Thomas Bykowski and Brian Stevenson, University of Kentucky College of Medicine

Unit 4.2 Culture of Escherichia coli and Related Bacteria
Sean P. Riley, Michael E. Woodman, and Brian Stevenson, University of Kentucky College of Medicine

Chapter 5 Sample Preparation

Unit 5.1 Centrifugation and Cell Fractionation In–House

Unit 5.2 Purification and Concentration of Nucleic Acids
Dennis H. Dowhan, University of Queensland

Chapter 6 Chromatography

Unit 6.1 Overview of Chromatography
Buddhadeb Mallik, Bulbul Chakravarti, and Deb N. Chakravarti, Keck Graduate Institute of Applied Life Sciences

Unit 6.2 Column Chromatography
Deb Chakravarti, Keck Graduate Institute

Chapter 7 Electrophoresis

Unit 7.1 Overview of Electrophoresis
Sean R. Gallagherr, UVP, LLC

Unit 7.2 Agarose Gel Electrophoresis
Jennifer A. Armstrong, The Claremont Colleges, and Joseph R. Schulz, Occidental College

Unit 7.3 SDS–PAGE
Sean Gallagher, UVP, LLC

Unit 7.4 Staining of Gels
Sean Gallagher, UVP, LLC

Chapter 8 Blotting

Unit 8.1 Overview of Blotting
Maria Cristina Negritto, Pomona College, and Glenn M. Manthey, City of Hope, Beckman Research Institute

Unit 8.2 Nucleic Acid Blotting: Southern and Northern
Laura L. Mays Hoopes, Pomona College

Unit 8.3 Protein Blotting: Immunoblotting
Sean Gallagher, UVP, LLC

Unit 8.4 Labeling DNA and Probe Preparation
Karl A. Haushalter, Harvey Mudd College

Chapter 9 Microscopy

Unit 9.1 Conventional Light Microscopy
Eric S. Cole, St. Olaf College

Unit 9.2 Immunofluorescence Microscopy
David J. Asai, Harvey Mudd College

Chapter 10 Enzymatic Reactions

Unit 10.1 Working with Enzymes
David Skrincosky and Cindy Santangelo, Worthington Biochemical Corp.

Unit 10.2 Overview of PCR
Christine D. Kuslich, Molecular Profiling Institute, Buena Chui, GE Healthcare, and Carl T. Yamashiro, Molecular Profiling Institute

Unit 10.3 Real–Time PCR
Dean Fraga, College of Wooster, Tea Meulia, Ohio Agricultural Research and Development Center, Steven Fenster, Ashland University

Chapter 11 Bioinformatics Unit 11.1 Using NCBI Blast
Nicholas A. Stover, Bradley University, and Andre R.O. Cavalcanti, Pomona College

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Wow! This is really a great book for anyone learning about or working in a research laboratory.   (Doody s, 30 August 2012)

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