Capillary Electrophoresis. Techniques in Analytical Chemistry

  • ID: 2174433
  • Book
  • 272 Pages
  • John Wiley and Sons Ltd
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The best source of practical, easily accessible information on this exciting new technique

CAPILLARY ELECTROPHORESIS

Analytical chemists and biochemists have been turning to the technique of capillary electrophoresis with increasing frequency: it is fast, sensitive, easy to automate, requires only small sample volumes and reagent amounts, and has been successfully applied to an ever–expanding list of sample types. In Capillary Electrophoresis, analytical practitioners will find a complete, practical guide to the principles, forms, and instrumentation of this technique. The book presents clear and straightforward explanations of the method, its operating principles, and its different modes, including capillary zone electrophoresis, micellar electrokinetic capillary chromatography, capillary gel electrophoresis, capillary isoelectric focusing, and capillary isotachophoresis. Especially helpful is the material on developing a method: it offers practical guidance on CE modes, capillaries, run buffers, voltage requirements, sample pretreatments, injection modes and amounts, temperature settings, detector selections, buffer replenishment, and data reporting.

Capillary Electrophoresis will serve both as an excellent introduction to those who are new to the technique and as a comprehensive reference book to experienced practitioners. A complete and detailed index will assist the reader in quickly finding any topic of interest.

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Principles of Separation.

Modes of Capillary Electrophoresis.

Instrumental Considerations.

Developing a Method.

Qualitative and Quantitative Analysis.

Applications.

Index.
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DALE R. BAKER is a consultant with expertise in capillary electrophoresis (CE) and high–performance liquid chromatography (HPLC). He has held positions with Dionex as a CE and HPLC applications scientist, and with DuPont and Hewlett–Packard in their HPLC laboratories. Dr. Baker holds a PhD in analytical chemistry from the University of Nebraska.
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