∗ Chromatographic procedures
∗ Measurement of 8–oxo deoxyguanosine
∗ Cellular–based methods
∗ Molecular–based assays
∗ Antioxidant activity.
Each part is designed to help the clinical scientist evaluate the best method for their particular problem in measuring oxidative damage in vivo. The methods represented are the ones most commonly used and are deemed robust and simple enough to apply to clinical material. Measuring in vivo Oxidative Damage is an ideal practical reference work for the clinical scientist and is a must for all laboratories in hospitals and research institutions which are actively involved in analytical work.
Lipid Peroxidation Determination by HPLC (R. Bevan).
Measurement of Oxidized Bases in DNA and Biological Fluids by Gas Chromatography Coupled to Mass Spectrometry (T. Douki, et al.).
The Measurement of Protein Oxidation by HPLC (H. Griffiths, et al.).
MEASUREMENT OF 8–OXO DEOXYGUANOSINE.
Measurement of 8–Oxo–2′ –deoxyguanosine in Cellular DNA by High Performance Liquid Chromatography–Electrochemical Detection (M. Evans).
Immunochemical Detection of 8–Oxodeoxyguanosine in DNA (M. Cooke & K. Herbert).
Urinary Measurement of 8–OxodG (8–Oxo–2′ –deoxyguanosine) (H. Poulsen, et al.).
Measurement of Oxidative DNA Damage Using the Comet Assay (A. Collins).
Measuring Oxidative DNA Damage by Alkaline Elution (M. Pflaum & B. Epe).
A ¯32P–Postlabelling Protocol to Measure Oxidative DNA Damage (G. Jones & M. Weinfeld).
Mapping Reactive Oxygen–Induced DNA Damage at Nucleotide Resolution (H. Rodriguez & S. Akman).
Measurements of Plasma Antioxidant Activity (S. Maxwell).