Microarray Quality Control

  • ID: 2178288
  • Book
  • 136 Pages
  • John Wiley and Sons Ltd
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Microarray technology provides researchers in the life sciences with a revolutionary tool for measuring gene expression. However, this highly developed process involves multiple steps, from sample selection to data analysis, each susceptible to potentially costly errors. Without sound quality control, experimental microarrays may produce useless or, even worse, misleading results.

Microarray Quality Control provides a comprehensive resource for ensuring quality control in every step of this complex process. From experimental design to data processing, analysis, and interpretation, the emphasis in this text remains on practical advice for each stage of planning and running a microarray study. Chapters cover:

  • Quality of biological samples
  • Quality of DNA
  • Hybridization protocols Scanning
  • Data acquisition
  • Image analysis
  • Data analysis

Written for the broad group of workers biologists, mathematicians, statisticians, engineers, physicians, and computational scientists involved in microarray studies, Microarray Quality Control features a straightforward style easily accessed by various disciplines. Useful checklists and tips help ensure the integrity of results, and each chapter contains a thorough review of pertinent literature.

The only complete, systematic treatment of the topic available, Microarray Quality Control offers students and practitioners an invaluable resource for improving experimental quality and efficiency.

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Foreword.

Preface.

1. Quality of Biological Samples.

1.1 Tissue Acquisition, Handling, and Storage.

1.2 Pathological Evaluation.

1.3 Tissue heterogeneity and Laser Capture Microdissection.

1.4 Summary.

2. Microarray Production: Quality of DNA and Printing.

2.1 Quality Control for cDNA Probes.

2.2 Long–oligo Arrays.

2.3 Slide Coating.

2.4 Slide Autofluorescence.

2.5 Printing Quality Control.

2.6 Summary.

3. Quality of Microarray Hybridization.

3.1 RNA and cDNA Labeling Quality.

3.2 Specificity versus Sensitivity.

3.3 Amplification Strategies.

3.4 Indirect Labeling.

3.5 Automation of Microarray Hybridization.

3.6 Hybridization Reference.

3.7 Validation of Microarray Experiments.

3.8 Summary.

4. Scanners and Data Acquisition.

4.1 Basic Principles of Scanners.

4.2 Basic Principles of Imagers.

4.3 Calibration and PMT Gain.

4.4 Characteristics of Different Noise Sources.

5. Image Analysis.

5.1 Grid Alignment.

5.2 Spot Segmentation.

5.3 Extracting Information.

5.4 Appendix: Image Processing.

6. Quality Control in Data Analysis.

6.1 Normalization.

6.2 Replication.

6.3 Missing Values.

6.4 Data Management Issues.

6.5 Small–Sample–Size Issues.

Index.

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Wei Zhang
Ilya Shmulevich
Jaakko Astola
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