Pocket Guide to Biotechnology and Genetic Engineering

  • ID: 2179566
  • Book
  • 360 Pages
  • John Wiley and Sons Ltd
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Biotechnology and genetic engineering are the key technologies of the 21st century. They allow the findings in cell biology and genetics, biochemistry and microbiology, biochemical engineering and bioinformatics to be applied to health care, agriculture, food production, environmental protection and alternative production methods for chemicals.

This handy book provides broad coverage of the relevant facts on products, methods and applications. It discusses the opportunities and risks involved in these new technologies, combined with ethical, economic and safety considerations. Instructive and attractive color illustrations as well as an excellent didactic approach throughout make this a perfect introduction to the field –– for professionals and students alike.
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INTRODUCTION

Early developments

Biotechnology today

BIOTECHNOLOGY OF FOOD

Alkoholic beverages

Beer

Fermentierted food

Food and lactic acid fermentation

ALCOHOLS, ACIDS AND AMINO ACIDS

Ethanol

1–Butanol, acetone

Acetic acid

Citric acid

Lactic acid and gluconic acid

Amino acids

L–Glutamic acid

D,L–Methionine, L–lysine, L–threonine

L–Phenylalanine and L–aspartic acid, Apartame?

Amino acids by enzymatic transformation

ANTIBIOTICS

Antibiotics: abundance, applications and mechanisms of action

Antibiotics: production and resistance

Lactam antibiotics: structure, biosynthesis and mechanism of action

Lactam antibiotics: production

Amino acid and peptide antibiotics

Glycopeptide, polyether and nucleoside antibiotics

Amino glycoside antibiotics

Chinones, chinolones and aromatic antibiotics

Polyketide antibiotics

New strategies for antibiotics

SPECIALTIES

Vitamins

Nukleosides and nucleotides

Biotensides and biocosmetics

Polysaccharides (dextran, xanthan)

Biomaterials

Biotransformation

Steroid–biotransformations

ENZYMES

Enzymes

Enzyme catalysis

Enzymes for analytics

Enzyme analytics

Enzymes in technical applications

Detergent enzymes

Enzymes für starch degradation

Enzymatic starch hydrolysis

Enzymes and sweetenting

Cellulasens and hemicellulases

Enzymes for pulp and paper production

Pectinases

Enzymes for milk treatment

Enzymes for treatment of bakery goods and meet

Enzymes for leather treatment

New startegies for technical enzymes

BAKERS′ YEAST AND FODDER YEASTS

Bakers′ yeast and fodder yeasts

Proteins and oil from singel cell organisms

BIOTECHNOLOGYIEUAND ENVIRONMENTAL PROTECTION

Aerobic wastewater treatment

Anaerobic wastewater and slugde treatment

Biological off–gas purification

Biological soil remediation

Microbial ore leaching and biocorrosion

THERAPEUTIC AND DIAGNOSTIC BIOTECHNOLOGY

Insulin

Somatotropin and ohter hormones

Hemoglobin, serum albumin, lactoferrin

Clotting factors

Anticoagulants and thrombolytics

Enzyme inhibitors

The immune system

Stem cells

Interferons

Interleukins

Erythropoietin and growth factors

TNF and other pharma proteins

Vaccines, recombinant vaccines

Antibodies

monoclonal antibodies

recombinant antibodies

Immune analytics

Biosensors

AGRICULTURAL BIOTECHNOLOGY

Animal breeding

Embryo transfer, cloned animals

Gen mapping

Transgenic animals

Gene farming

Plant breeding

Plant cell cultures: surface cultures

Plant cell cultures: suspension cultures

Transgenic plants: methods

Transgenic plants: resistance

Transgenic plants as bioreactors

MICROBIOLOGICAL FUNDAMENTALS

Viruses

Bakteriophags

Microorganisms

Bacteria

Bacteria: charakterization

Fungi

Yeasts

Microorganisms: isolation, stem maintenance, safety

Stem maintenance of microorganisms

FUNDAMENTALS OF BIOPROCESS ENGINEERING

Culture

growth kinetics and product formation

fed–batch and continuous fermentations

Fermentation technique

Fermentation technique: upscaling

Cultivation of animal cells

Cultivation of animal cells: technical concepts

Enzyme and cell reactors

Downstream processing of bioproducts

Downstream processing of bioproducts: chromatography

Industrial processes

MOLECULAR GENETIC METHODS

DNA: structure

DNA: Function

Gene technology: general working steps

Preparation of DNA, restriction enzymes

Enzymes fortreatment of DNA

PCR – general method and applications

PCR DNA: synthesis and size determination – application examples

DNA: sequencing

Introduction of DNA in living cells (transformation)

Cloning and identificationof genes

Gene expression

Silencing of genes

Protein design

Gen banks and gene mapping

Genomes of prokaryotes

Genomes of eukaryotes

Human genome

Functional analysis of the human genome

DNA analytics

DNA arrays

Reporter groups

Gene therapy

CURRENT TRENDS

Compund screening

Proteomics

Bioinformatics

Metabolism

Metabolic engineering

SAFETY, ETHICS, ECONOMY

Safety in gene technology

Approval of bio– and gene technicologiocal products

Ethics and acceptance

Patents and biotechnology

International comparison
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Rolf D. Schmid
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