+353-1-416-8900REST OF WORLD
+44-20-3973-8888REST OF WORLD
1-917-300-0470EAST COAST U.S
1-800-526-8630U.S. (TOLL FREE)

PRINTER FRIENDLY

Production of Recombinant Proteins. Novel Microbial and Eukaryotic Expression Systems. Edition No. 1

  • ID: 2179584
  • Book
  • December 2004
  • 429 Pages
  • John Wiley and Sons Ltd
While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section -- with strains, genetic elements, vectors and special methods, where applicable -- as well as examples of proteins produced with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems -- mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B vaccine chosen as an industrial example.

With a foreword by Herbert P. Schweizer, Colorado State University, USA:
"As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."
Note: Product cover images may vary from those shown
Foreword by Herbert P. Schweizer, Colorado State University, USA

Introduction

Key and Criteria to the Selection of an Expression Platform

BACTERIAL SYSTEMS
Escherichia coli
Pseudomonas fluorescens
Staphylococcus carnosus and other Gram-positive Bacteria

YEAST SYSTEMS
Arxula adeninivorans
Hansenula polymorpha
Pichia pastoris
Yarrowia lipolytica

FILAMENTOUS FUNGI
Aspergillus sojae
Sordaria macrospora

EUKARYOTIC CELLS
Mammalian Cells
Plant Cells

Wide-Range Integrative Expression Vectors for Fungi, Based on Ribosomal DNA Elements
Comparative Fermentation
Recombinant Hepatitis B Vaccines: Disease Characterization and Vaccine Production
Biopharmaceuticals and the Industrial Environment
Note: Product cover images may vary from those shown
Gerd Gellissen Aachen, Germany.
Note: Product cover images may vary from those shown
Adroll
adroll