Production of Recombinant Proteins. Novel Microbial and Eukaryotic Expression Systems

  • ID: 2179584
  • Book
  • 429 Pages
  • John Wiley and Sons Ltd
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While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section – with strains, genetic elements, vectors and special methods, where applicable – as well as examples of proteins expressed with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram–negatives and one Gram–positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems – mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial–scale fermentation, with the production of a recombinant Hepatitis B B vaccine chosen as a n industrial example.

With a foreword by Herbert P. Schweizer, Colorado State University, USA: "As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."

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Foreword by Herbert P. Schweizer, Colorado State University, USA

Introduction

Key and Criteria to the Selection of an Expression Platform

BACTERIAL SYSTEMS

Escherichia coli

Pseudomonas fluorescens

Staphylococcus carnosus and other Gram–positive Bacteria

YEAST SYSTEMS

Arxula adeninivorans

Hansenula polymorpha

Pichia pastoris

Yarrowia lipolytica

FILAMENTOUS FUNGI

Aspergillus sojae

Sordaria macrospora

EUKARYOTIC CELLS

Mammalian Cells

Plant Cells

Wide–Range Integrative Expression Vectors for Fungi, Based on Ribosomal DNA Elements

Comparative Fermentation

Recombinant Hepatitis B Vaccines: Disease Characterization and Vaccine Production

Biopharmaceuticals and the Industrial Environment
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Gerd Gellissen
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