Electrophoresis in Practice. 4th, Revised and Updated Edition

  • ID: 2179612
  • Book
  • 426 Pages
  • John Wiley and Sons Ltd
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This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use.

This fourth edition retains the successful concept of its predecessors, yet features a brand–new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references.
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PART I: FUNDAMENTALS

Introduction

Electrophoresis

Isotachophoresis

Isoelectric Focusing

Blotting

Interpretation of Electropherograms

Proteome Analysis

Instrumentation

PART II: EQUIPMENT AND METHODS

Equipment for Part II

Method 1: PAGE of Dyes

Method 2: Agarose and Immuno Electrophoresis

Method 3: Titration Curve Analysis

Method 4: Native PAGE in Amphoteric Buffers

Method 5: Agarose IEF

Method 6: PAGIEF in Rehydrated Gels

Method 7: Horizontal SDS–PAGE

Method 8: Vertical PAGE

Method 9: Semi–dry Blotting of Proteins

Method 10: IEF in Immobilized pH Gradients

Method 11: High–resolution 2D Electrophoresis

Method 12: PAGE of Double Stranded DNA

Method 13: Native PAGE of Single Stranded DNA

Method 14: Denaturing Gradient Gel Electrophoresis

Method 15: Denaturing PAGE of DNA

Appendix A: Trouble–shooting

References

Index
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From the reviews of previous editions:


"The illustrations are informative and to the point...The book clearly illustrates the competence of the author. He generously shares his experience and knowledge with the reader, especially in the field of gel–based electrophoresis...an excellent reference to help the reader to become acquainted with the field of electrophoresis. The book contains theoretical and ample practical information that serves not only the practician in the electrophoresis laboratory, but also students and lecturers in electrophoretic methods." (Clinical Chemistry)

"The rigorous description of each method along with the extensive figures will easily allow the novice to reproduce these methods in the laboratory" (The Analyst)

"An excellent book which we recommend greatly and which has not to be missed in any laboratory of cellular and molecular biology which respects itself." (Cellular and Molecular Biology)

"As a comprehensive guide to the huge variety of electrophoretic methods now available, this is very good value. The superb troubleshooting appendix almost justifies the price on its own." (Laboratory Equipment Digest)
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