Electrophoresis in Practice. 4th, Revised and Updated Edition

  • ID: 2179612
  • Book
  • 426 Pages
  • John Wiley and Sons Ltd
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This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use.

This fourth edition retains the successful concept of its predecessors, yet features a brand–new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references.
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PART I: FUNDAMENTALS

Introduction

Electrophoresis

Isotachophoresis

Isoelectric Focusing

Blotting

Interpretation of Electropherograms

Proteome Analysis

Instrumentation

PART II: EQUIPMENT AND METHODS

Equipment for Part II

Method 1: PAGE of Dyes

Method 2: Agarose and Immuno Electrophoresis

Method 3: Titration Curve Analysis

Method 4: Native PAGE in Amphoteric Buffers

Method 5: Agarose IEF

Method 6: PAGIEF in Rehydrated Gels

Method 7: Horizontal SDS–PAGE

Method 8: Vertical PAGE

Method 9: Semi–dry Blotting of Proteins

Method 10: IEF in Immobilized pH Gradients

Method 11: High–resolution 2D Electrophoresis

Method 12: PAGE of Double Stranded DNA

Method 13: Native PAGE of Single Stranded DNA

Method 14: Denaturing Gradient Gel Electrophoresis

Method 15: Denaturing PAGE of DNA

Appendix A: Trouble–shooting

References

Index
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Reiner Westermeier
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